大鼠正畸复发过程中牙周组织IL-6表达的研究

doi:10.13701/j.cnki.kqyxyj.2016.11.010

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (11): 1153-1155.DOI: 10.13701/j.cnki.kqyxyj.2016.11.010

大鼠正畸复发过程中牙周组织IL-6表达的研究

高睿,安晶涛,宋冰,邵坪^*

哈尔滨医科大学口腔医学院正畸科黑龙江哈尔滨 150001

收稿日期:2016-04-29 出版日期:2016-11-25 发布日期:2016-11-25
通讯作者: 邵坪,E-mail:13604881927@163.com
作者简介:高睿(1982~ ),男,哈尔滨人,硕士在读,主治医师,主要从事口腔正畸的临床治疗工作。

Expression of IL-6 in Periodontal Tissue during Orthodontic Relapse in Rats

GAO Rui, AN Jing-tao, SONG Bing, SHAO Ping^*

Department of Orthodontics, College of Stomatology, Harbin Medical University, Harbin 150001, China

Received:2016-04-29 Online:2016-11-25 Published:2016-11-25

摘要/Abstract

摘要： 目的:研究在正畸复发的过程中,大鼠牙周组织中炎症因子白细胞介素IL-6表达的变化。方法:选取30只Wistar雄性大鼠,随机分为正常对照组(6只)与正畸牙齿移动模型组(24只),模型组在加力14 d后去除装置,并随机分为模型0 d组、模型7 d组、模型14 d组、模型21 d组,分别在去除装置的第0天、第7天、第14天、第21天处死实验动物,取牙周组织,使用Western Blot和RT-PCR法检测炎症因子IL-6蛋白及基因水平表达的变化。结果:与对照组相比,模型0 d组的IL-6的蛋白和基因表达水平并没有明显变化。但模型7 d组的IL-6的蛋白和基因表达水平显著增加并达到了IL-6表达的最高值(P<0.05)。模型14 d组的IL-6表达水平虽有下降但也明显高于对照组(P<0.05)。而与模型7 d组相比,模型21 d组的IL-6蛋白和基因表达水平显著降低(P<0.05),并接近对照组水平(P>0.05)。结论:大鼠正畸复发导致IL-6蛋白及基因的表达水平显著增加,但在正畸复发的第14天开始逐渐消退减少。

关键词: 正畸复发, 正畸牙齿移动模型, 白细胞介素-6, 牙周组织

Abstract: Objective: To study the expression of inflammatory cytokines interleukin IL-6 in rat periodontal tissues during orthodontic relapse. Methods: Thirty male Wistar rats were randomly divided into normal control group (6) and orthodontic tooth movement model group (24). Devices of the model group were removed after 14 days with rats randomly divided into model-0 day, model-7 day, model-14 day, and model-21 day group. Animals were sacrificed on day 0, day 7, day 14 and day 21, respectively. Then, periodontal tissues were obtained to detect the changes of inflammatory cytokines IL-6 in protein and gene expression levels. Results: Compared with the control group, protein and gene expression levels of IL-6 in Model-0 day group did not change significantly (P>0.05). But the expression level of IL-6 protein and gene on Model-7 day increased significantly and reached the highest (P<0.05). The expression level of IL-6 protein and gene on Model-14 day was higher than the control group, but a little lower than the Model-7 day. The expression level of IL-6 protein and gene on Model-21 day significantly decreased when compared to the Model-7 day, and was close to the control group (P>0.05). Conclusion: The expression levels of IL-6 protein and gene significantly increased in the condition of rat orthodontic relapse, arrived the top level on Model-14 day, and then decreased gradually.

Key words: Orthodontic relapse, Orthodontic tooth movement, Interleukin-6, Periodontal tissue

中图分类号:

R783.5

高睿,安晶涛,宋冰,邵坪. 大鼠正畸复发过程中牙周组织IL-6表达的研究[J]. 口腔医学研究, 2016, 32(11): 1153-1155.

GAO Rui, AN Jing-tao, SONG Bing, SHAO Ping. Expression of IL-6 in Periodontal Tissue during Orthodontic Relapse in Rats[J]. Journal of Oral Science Research, 2016, 32(11): 1153-1155.

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大鼠正畸复发过程中牙周组织IL-6表达的研究

Expression of IL-6 in Periodontal Tissue during Orthodontic Relapse in Rats

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