种植体穿龈部分氮化钛纳米涂层对牙龈成纤维细胞粘附和增殖的影响

doi:10.13701/j.cnki.kqyxyj.2017.02.009

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (2): 154-157.DOI: 10.13701/j.cnki.kqyxyj.2017.02.009

种植体穿龈部分氮化钛纳米涂层对牙龈成纤维细胞粘附和增殖的影响

潘夏薇¹, 赖颖真¹, 陈江^2*

1. 厦门医学高等专科学校福建厦门 361000;
2. 福建医科大学附属口腔医院种植科福建福州 350000

收稿日期:2016-08-06 出版日期:2017-02-20 发布日期:2017-02-22
通讯作者: 陈江,电话:18659260100
作者简介:潘夏薇(1984~ ),女,福建漳州人,硕士在读,讲师,主要从事口腔种植教学与科研工作。
基金资助:
福建省临床重点专科建设项目(编号:20121589)

Effect of TiN Nano-coating on Adhesion and Proliferation of Human Gingival Fibroblasts in Transmucosal Part of Dental Implant.

PAN Xia-wei¹, LAI Ying-zhen¹, CHEN Jiang^2*

1. Xiamen Medical College, Xiamen 361000, China;
2. Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350002, China

Received:2016-08-06 Online:2017-02-20 Published:2017-02-22

摘要/Abstract

摘要： 目的:明确氮化钛纳米(TiN)涂层对人牙龈成纤维细胞(HGF)粘附形态和增殖的影响, 作为选择合适的种植体穿龈部分涂层的依据之一。方法:利用磁控溅射技术制作氮化钛(TiN)和对照Ti涂层,测量各组材料表面形貌、元素含量、粗糙度和亲水性,原代培养人牙龈成纤维细胞(HGF),通过扫描电镜SEM和CCK-8观察分析不同纳米涂层上方细胞粘附和增殖差异。结果:TiN组表面HGF粘附形态与Ti组相似,而HGF增殖幅度大于对照Ti。结论:本实验所设计的氮化钛(TiN)涂层利于促进HGF增殖,推荐作为种植体穿龈部分涂层。

关键词: 软组织, 种植体, 氮化钛(TiN)纳米涂层, 人牙龈成纤维细胞(HGF), 细胞增殖

Abstract: Objective: To investigate the effect of TiN nano-coating on adhesion and proliferation of the human gingival fibroblasts (HGF). Methods: TiN coatings were deposited by magnetron sputtering. Ti coatings were deposited as the controls. The morphology and roughness of nano-coatings, elementary composition and hydrophilia were determined. Morphology of HGF was analyzed by using SEM. Cell proliferation abilities were investigated by using CCK-8 Kit. Results: Morphology of HGF was similar between TiN coatings and the control. However, the cell proliferation ability was significantly increased in substrate with TiN nano-coating. Conclusion: TiN nano-coating can be applied in transmucosal part of dental implant to enhance the biocompatibility.

Key words: Soft tissue Implant , TiN nano-coating , Human gingival fibroblasts , Proliferation

中图分类号:

R782.12

潘夏薇, 赖颖真, 陈江. 种植体穿龈部分氮化钛纳米涂层对牙龈成纤维细胞粘附和增殖的影响[J]. 口腔医学研究, 2017, 33(2): 154-157.

PAN Xia-wei, LAI Ying-zhen, CHEN Jiang. Effect of TiN Nano-coating on Adhesion and Proliferation of Human Gingival Fibroblasts in Transmucosal Part of Dental Implant.[J]. Journal of Oral Science Research, 2017, 33(2): 154-157.

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种植体穿龈部分氮化钛纳米涂层对牙龈成纤维细胞粘附和增殖的影响

Effect of TiN Nano-coating on Adhesion and Proliferation of Human Gingival Fibroblasts in Transmucosal Part of Dental Implant.

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