Analysis on the Diversity of Salivary Microbial of Adult Dental Caries Based on 16S Ribosomal RNA Clone Library.

doi:10.13701/j.cnki.kqyxyj.2016.03.015

Abstract

Abstract: Objective: To discuss the difference of saliva microbial structure between the caries-active and caries-free adult population. Methods: According to the WHO sampling standard, 6 saliva samples were collected with 3 caries-active (CA) subjects and 3 caries-free (CF) subjects. DNA of the saliva samples was extracted and the 16S ribosomal RNA gene clone library was constructed. The positive clones were sequenced and analyzed by software. The phylogenetic tree was built by MEGA 4.0. Results: A total of 87 OTUs were obtained and all of the OTUs were devided into 5 phyla, 9 classes, 10 orders, 14 families and 19 genera. The dominant genera in CA group included: Streptococcus (53.16%), Prevotella (28.77%) and Granulicatella (9.34%). The dominant genera in CF group included: Streptococcus (46.12%), Prevotella (23.41%) and Neisseria (14.35%). Conclusion: As 16S ribosomal RNA gene clone library method has already matured, it can be used to study oral microbial community structure. There are differences between the Han caries-active and caries-free population. The specific mechanism of the dominant genera in CA needs further research.

Key words: Oral microorganisms , Oral cavity , Dominant bacteria 16S, rRNA , Clone library

CLC Number:

R781.1

LI Jun-ping, WU Fang, WANG Zhen-zhen, WANG Yi-bin, ZHOU Jian-ye, LI Zhi-qiang, YU Zhan-hai. Analysis on the Diversity of Salivary Microbial of Adult Dental Caries Based on 16S Ribosomal RNA Clone Library.[J]. Journal of Oral Science Research, 2016, 32(3): 272-277.

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