Effects of Sema3A on the Proliferation and Mineralization of a Cementoblast Cell Line OCCM-30.

doi:10.13701/j.cnki.kqyxyj.2016.05.005

Journal of Oral Science Research ›› 2016, Vol. 32 ›› Issue (5): 445-448.DOI: 10.13701/j.cnki.kqyxyj.2016.05.005

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Effects of Sema3A on the Proliferation and Mineralization of a Cementoblast Cell Line OCCM-30.

QIU Yu^1,2, REN Ai-shu^2,3, PANG Qin^1,2, MA Xiao-juan^1,2, FU Gang^1,4*.

1. Department of Implant Dentistry, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China;
2. Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China;
3. Department of Orthodontics, Affiliated Hospital of Stomatology, Chongqing Medical University , Chongqing 401147, China;
4. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China

Received:2015-09-16 Online:2016-05-26 Published:2016-05-26

Abstract

Abstract: Objective: To investigate the effects of Semaphorin3A (Sema3A) on the proliferation and mineralization of cementoblasts. Methods: The immortalized murine cementoblast cell line OCCM-30 was treated with recombinant human Sema3A (0, 0.5, 1mg/L). Cell proliferation was tested by MTT assay. Alkaline phosphotase (ALP) activity was detected using an ALP kit. The mRNA levels of osteocalcin (OCN), osteopontin (OPN) and Runx2 were investigated by quantitative reverse transcription PCR (RT-PCR). Calcium deposition was assayed with Alizarin red staining (ARS). Results: The proliferation and the ALP activity of OCCM-30 treated with Sema3A were not significantly different from those of the control. The OCN and Runx2 mRNA expressions of the OCCM-30 treated with Sema3A significantly declined in contrast to that of the control. Sema3A also inhibited the mineral nodule formation in vitro compared to untreated cells. Conclusion: Sema3A did not obviously affect the proliferation, but suppressed the mineralization of the cementoblasts.

CLC Number:

R783.4

QIU Yu, REN Ai-shu, PANG Qin, MA Xiao-juan, FU Gang.. Effects of Sema3A on the Proliferation and Mineralization of a Cementoblast Cell Line OCCM-30.[J]. Journal of Oral Science Research, 2016, 32(5): 445-448.

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Effects of Sema3A on the Proliferation and Mineralization of a Cementoblast Cell Line OCCM-30.

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