Abstract: Objective: To investigate effects of experimentally altered anterior guidance on the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in temporomandibular joint synovium of rats. Methods: Anterior guidance deletion model was induced by eliminating the contact between anterior teeth in every direction with a diamond wheel. The anterior guidance accretion models were accomplished by cemented metal crowns which gained 15 and 30 degree on palatal side to the incisors of rats’ maxilla. Two animals in each group were sacrificed after 3, 7, 14, and 28 days. The complete TMJ of one side and TMJ synovium of the other side were obtained for analysis of iNOS and COX-2 expression by immunohistochemistry (IHC) and real-time polymerase chain reaction analysis (real-time PCR). Results: The anterior guidance in 30 degree group (group T3) showed statistical differences when compared with control group (group C) in COX-2 and iNOS mRNA expression



基金项目 陕西省社会发展科技攻关基金(编号:2012k16-10-03)
作者简介 姚天华(1976~ ),女,河南人,硕士,主治医师, 主要从事口腔综合类研究。
李雨轩(1988~ ),女,陕西人,硕士,医师,主要研究方向为牙合学与颞下颌关节紊乱病。
*通讯作者 肖敏,电话:029-87218541
#为共同第一作者
(P<0.05). In group T3, the level of COX-2 reached the highest on day 3 and there was statistical difference among specimens sacrificed after 3d, 7d and 28d (P<0.008). iNOS expression peaked on day 7, but showed no statistical differences compared with those of day 3, 14, and 28. Conclusion: COX-2 and iNOS may be involved in TMJ synovitis caused by altered anterior guidance.

Key words: Anterior, guidance, Animal, Model, Temporomandibular, joint, synovium, COX-2, iNOS