Abstract: Objective: To prepare biphasic calcium phosphate scaffolds for bone tissue engineering by selective laser sintering and to evaluate the biocompatibility of the scaffolds. Methods: After fully mix of biphasic calcium phosphate powders and epoxy resin powders, the green parts of biphasic calcium phosphate scaffolds were fabricated by a selective laser sintering machine. Then the epoxy resin was burned during sintering in muffle furnace to get final scaffolds. X-ray diffraction and Fourier transform infrared spectroscopy were used to examine the composition of scaffolds. The scaffolds surface was observed by scanning electron microscope. The morphology of MC3T3-E1 cells adhered on scaffolds was observed by confocal laser scanning microscope after culture for 7 days. CCK-8 assay and living-dead staining were performed to evaluate the cell growth. Results: After sintering in muffle furnace, the remained epoxy resin was removed. The porous structure could be found on the surface of scaffolds. MC3T3-E1 cells could distribute well on the surface of scaffolds. The result of CCK-8 assay showed that there was no significant differences on cell viability between cells cultured in scaffolds and in culture plates at day 10. Conclusion: Selective laser sintered biphasic calcium phosphate not only possesses porous structure but also has good biocompatibility.

Key words: Selective laser sintering, Biphasic calcium phosphate, MC3T3-E1 cells, Bone tissue engineered scaffolds