Abstract: Objective: To evaluate the cytotoxicity of Fufang Bingpeng Irrigants, Bingpeng Irrigants,sodium hypochloride,jinzhi gargle, and chlorhexidine gluconatie compound gargle on mouse fibroblasts L929. Methods: L929 fibroblasts were cultured in vitro. Cells were cultured with five irrigants for 6 hours, 12 hours, 24 hours, and 48 hours. The culture medium was set as the control group. The MTT assay was employed to evaluate the cytotoxicity. Results: Higher concentrations of irrigants generally showed significantly stronger inhibitory effects. Fufang Bingpeng Irrigants and Bingpeng Irrigants showed no cytotoxicity,jinzhi gargle showed middle, while sodium hypochloride and chlorhexidine displayed higher toxicity. The rank of the irrigation cytotoxicity from the most to the least was: chlorhexidine, sodium hypochloride, jinzhi gargle, Bingpeng Irrigants, and Fufang Bingpeng Irrigants. Conclusion: The Fufang Bingpeng Irrigants showed no detectable cytotoxicity. As a root canal irrigant, Chinese medicine alteratives might be adopted.

Key words: Fufang Bingpeng irrigants, Root canal irrigant, Cytotoxicity, L929 fibroblasts