RNA-Seq Analysis of Temporal and Spatial Expression of Genes in the Key Period of Palatogenesis in Mice.

doi:10.13701/j.cnki.kqyxyj.2018.12.022

Abstract

Abstract: Objective: To construct DEGB during palatogenesis in mice, and to explore the key regulatory factors and regulation mechanism of palate development.Methods: The embryonic palatal shelves of pregnant C57BL/6 at Ed13.5, Ed14.5, Ed15.5, and Ed16.5 were collected respectively for RNA-seq to screen DEGs.Selected DEGs were validated and analyzed by qPCR and immunohistochemistry.Results: RNA-seq identified 243 DEGs (27 DEGs were down-regulated and 216 DEGs were up-regulated) at the first stage of palatal development (Ed13.5-Ed14.5), 208 DEGs (30 DEGs down-regulated and 178 DEGs up-regulated) at the second stage (Ed14.5-Ed15.5), and 262 DEGs between E15.5 and Ed16.5 (31 DEGs down-regulated and 231 DEGs up-regulated) at the third stage (Ed15.5-Ed16.5).The expression of DEGs related with micro RNA and crystallin family changed significantly.Immunohistochemistry showed that Spp1 was gradually increased in palatal mesenchyme from Ed13.5 to Ed16.5.Conclusion: Members in micro RNA family and crystallin family and Spp1 gene play a role in the palatogenesis.

Key words: Palate development, Cleft lip and palate, RNA-Seq

PENG Yao, SU Chao-nan, WANG Xin-huan, WANG Kun, QIAO Wei-wei, GAO Qian, MENG Liu-yan. RNA-Seq Analysis of Temporal and Spatial Expression of Genes in the Key Period of Palatogenesis in Mice.[J]. Journal of Oral Science Research, 2018, 34(12): 1356-1359.

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