Deletion of Bmp2 and Bmp4 from Epithelium Affected Tooth Root Development in Mice

doi:10.13701/j.cnki.kqyxyj.2020.08.006

Abstract

Abstract: Objective: To observe the effect of double deletion of Bmp2 and Bmp4 from epithelium on root development, which provide a preliminary basis for further study of molecular mechanism. Methods: The primers recommended by Jackson Laboratory were used to identify the genotype of the mice. The mandibles were prepared for HE staining. Morphological analyses of hertwig's epithelial root sheath (HERS) and root odontoblast cells were conducted in the Bmp2 and Bmp4 knockout mice and the control mice during root development. The roots from the control and DcKO mice were analyzed by plain X-ray radiography. Results: At postnatal 0 day (PN0), the roots did not develop yet. At PN7, the polarization of odontoblasts in DcKO mice was worse compared to that of the normal controls. At PN14, the terminal HERS had fragmented. In the DcKO group, the terminal HERS was more difficult to fragment. At PN21 and PN28, plain X-ray images indicated that the root length was significantly reduced and the root pulp cavity was increased in the DcKO mice. Conclusion: Bmp2 and Bmp4 genes are involved in the root development of mice. The deletion of Bmp2 and Bmp4 genes leads to shorter roots in the DcKO mice in contrast to control mice.

Key words: Bmp2, Bmp4, mice, root development

GENG Shuoshuo, LIU Xin, GUO Shouli, LI Ying, GUAN Xue, WANG Xiumei, XIE Xiaohua. Deletion of Bmp2 and Bmp4 from Epithelium Affected Tooth Root Development in Mice[J]. Journal of Oral Science Research, 2020, 36(8): 726-730.

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