Preliminary Study on Therapeutic Effect of AE-PDT on Tongue Squamous Cell Carcinoma in Nude Mice.

doi:10.13701/j.cnki.kqyxyj.2017.12.007

Abstract

Abstract: Objective: To investigate the efficacy of aloe-emodin (AE)-mediated photodynamic therapy (PDT) in the treatment of human tongue squamous cell carcinoma, and to provide a theoretical basis for clinical treatment. Methods: Tca-8113 cells were implanted into nude mice subcutaneously in 4 groups (control group, light group, AE group, and PDT group). After treatment, the tumor volume was measured periodically. After 14 days, three mice were sacrificed and the tumor growth rate was calculated in each group. The survival time was observed and the life extension rate was calculated in the remaining mice. Results: The tumor volume of PDT group was significantly higher than that of control group (P<0.05). The tumor inhibition rates of light group, AE group, and PDT group were 4.76%, 20.24%, and 77.40% respectively. The results showed the tumor necrosis and vacuolar degeneration in PDT group which were not obviously detected in the control group and the light group. All treatments could prolong the survival time of the mice, but the PDT group exhibited the longest survival time and the highest rate of extension. Conclusion: AE-PDT can inhibit the growth of tongue squamous cell carcinoma and prolong the survival time of tumor mice.

Key words: Aloe emodin , Photodynamic therapy, Tongue squamous cell carcinoma , Nude mice

CLC Number:

R739.8

ZHANG Hong-chao, LIU Yun-qing, SHAO Ming-jing, WANG Cui-ping, BI Liang-jia, MENG Pei-song.. Preliminary Study on Therapeutic Effect of AE-PDT on Tongue Squamous Cell Carcinoma in Nude Mice.[J]. Journal of Oral Science Research, 2017, 33(12): 1266-1269.

References

[1] Kamali A,Gahm C,Palmgren B,et al. Regional recurrence in early stage I-II oral tongue cancer: a single institutional study and review of the literature [J]. Acta Otolaryngol,2017,22(1)∶1-7
[2] 田鑫,莫立根,罗莹.临床N0期舌癌颈淋巴结转移相关因素及其外科处理[J].肿瘤防治研究,2014,41(7)∶751-755
[3] Mfouo-Tyngal, and Abrahamse H. Cell death pathways and phthalocyanine as an efficient agent for photodynamic cancer therapy [J]. Int J Mol Sci, 2015, 16(5)∶10228-10241
[4] Kangdong Liu, Chanmi Park, Li S, et al. Aloe-emodin suppresses prostate cancer by targeting the mTOR complex2 [J]. Carcinogenesis,2012,33(7)∶1406-1411
[5] Hao CH, Wang WT, Song SH, et al. Establishment of rat coro-nary thrombosis model by photochemistry [J]. Chin PharmacolBull, 2012, 28(1)∶143-147
[6] Shirasu N, Nam So, Kuroki M. Tumor-targeted photodynamic therapy [J]. Anticancer Rws,2013,33(7)∶2823-2831
[7] Hu B,Zhang H,Meng X,et al. Aloe-emodin from rhubarb (Rheum rhabarbarum) inhibits lipopolysaccharide induced inflammatory respon-ses in RAW264.7 macrophages [J]. Ethnopharmacol,2014,153(3)∶846-853
[8] Richard MM, John AO, Paul CO, et al. M ethanolic extracts of aloe secundiflora engl inhibits in vitro growth of tuberculosis and diarrhea-causing bacteria [J]. P harm acognosy Res,2011,3(2)∶95-99
[9] Planka J, Latocha M. Photodynamic therapy in the treatment of breast cancer [J]. Pol Merkur Lekarski,2012,33(195)∶173-175.
[10] 刘琼瑶,廖鹏程,谭红,等.大鼠舌黏膜癌变过程中E-cad、CD44s的表达[J].口腔医学研究,2017,33(2)∶158-161
[11] Khangura SK,Greenwald BD. Endoscopic management of esophageal cancer after definitive chemoradiotherapy [J]. Dig Dis Sci,2013, 58(6)∶1477-1485
[12] Pecere T, Gazzola MV, Mucignat C, et al. Aloe-emodin Is a New Type of Anticancer Agent with Selective Activity against Neuroectodermal Tumors [J]. Cancer Res,2000,60(11)∶2800-2804

[1]	PEI Xiyan, SUN Feng. Research Progress on Minimally Invasive Non-surgical Periodontal Therapy [J]. Journal of Oral Science Research, 2020, 36(7): 622-625.
[2]	LI Tianke, ZHANG Ying, ZHANG Suxin, CHEN Zhong, BAO Yang, ZHANG Guanhua. Identification of Hub Genes in Tongue Squamous Cell Carcinoma Based on Weighted Gene Co-expression Network Analysis [J]. Journal of Oral Science Research, 2020, 36(5): 423-427.
[3]	WEI Diyuan, YAN Zhimin. Research Progress and Application Potential of Photodynamic Therapy in Treating Oral Candidiasis [J]. Journal of Oral Science Research, 2020, 36(12): 1087-1090.
[4]	ZHOU Qun, QIU Jiaxuan. Plumbagin Induces Tongue Squamous Cell Carcinoma Cells Apoptosis via ROS-mediated JNK Signaling Pathway [J]. Journal of Oral Science Research, 2020, 36(10): 921-924.
[5]	DENG Xuan, YANG Fang, TANG Xiqing. Effect of Down-regulation of Mucin-1 on Growth of Xenografts of Human Tongue Squamous Cell Carcinoma Tca-8113 in Nude Mice [J]. Journal of Oral Science Research, 2019, 35(8): 776-780.
[6]	WANG Rui, LI Li-heng, XU Ai-wen, AN Feng. Lentivirus Mediated Down-regulation of Med19 Expression Enhances Sensitivity of Tongue Squamous Cell Carcinoma to Cisplatin [J]. Journal of Oral Science Research, 2019, 35(6): 559-562.
[7]	ZHANG Xin-yu, WU Shuang-yan, WU Hong, XU Zhu-qing, WANG Yun-ying, XU Xiao-na, LI Xin, ZHENG Jian-jin. Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma. [J]. Journal of Oral Science Research, 2019, 35(5): 443-447.
[8]	CHEN Chang, PENG Yong-jian, CHEN Yan. Effects of eIF4E-siRNA on Adhesion and Movement Ability of Tongue Squamous Cell Carcinoma Cells [J]. Journal of Oral Science Research, 2019, 35(1): 51-55.
[9]	SHAO Ming-jing,WANG He, DONG Li, ZHANG Hong-chao, LIU Xu, MENG Pei-song.. Therapeutic Effect of Aloe-emodin Nanometer Liposome-mediated Photodynamic Therapy on Human Tongue Squamous Cell Carcinoma Tca-8113 Cells. [J]. Journal of Oral Science Research, 2018, 34(9): 948-951.
[10]	DAI Meng-ying, ZHANG Yang, ZHANG Zhao-nan, SU Wei-peng, ZHANG Song-an, LIU Pan, ZHAO Hua-rong. Effects of Long Non-coding RNA HOTAIR on Proliferation, Migration, and Invasion of Tongue Squamous Cell Carcinoma [J]. Journal of Oral Science Research, 2018, 34(6): 627-631.
[11]	YANG Hai-li, ZOU Hai-xiao, SONG Li. Research Progress in Photodynamic Therapy for Peri-implantitis. [J]. Journal of Oral Science Research, 2018, 34(5): 467-469.
[12]	FENG Tie-jun, WANG Yu-dong, PAN Xuan, CHI Yu-feng. Effect of Transfection of DEK Gene Small Interference RNA on Proliferation and Apoptosis of Tongue Squamous Cell Carcinoma. [J]. Journal of Oral Science Research, 2018, 34(4): 414-418.
[13]	HUANG Yu-si, ZHANG Lei, LIU Le-tian, MA Yao, LIU Xin. Effects of DVDMS-PDT on Proliferation and Migration of Human Tongue Squamous Carcinoma Cell Line HSC-4 [J]. Journal of Oral Science Research, 2018, 34(11): 1208-1211.
[14]	ZHOU Yan-meng, LANG Chun-mei, SHEN Hang, HU Fang-yuan, HU Ming, DENG Wen-bo.. Effects of Two-dimensional and Three-dimensional Culture on Biological Characteristics of Human Tongue Squamous Cell Carcinoma TCA8113 Cell Lines. [J]. Journal of Oral Science Research, 2018, 34(10): 1122-1126.
[15]	LIU Yu-lan, MENG Lin, TANG Qi, BU Wen-huan, HUANG Guo-shuai, DAI Yi-bin, LIU Qi-lin, SUN Hong-chen. Ascorbic Acid Carbon Dots Kill Tongue Squamous Cell Carcinoma Cell by Promoting Autophagy [J]. Journal of Oral Science Research, 2018, 34(1): 35-38.