Expression of miR-30d in Tongue Squamous Cell Carcinoma and Effect of Antisense miR-30d on Cell Proliferation and Invasion.

doi:10.13701/j.cnki.kqyxyj.2017.12.006

Abstract

Abstract: Objective: To investigate the expression of miR-30d in tongue squamous cell carcinoma (TSCC) and effect of antisense miR-30d on cell proliferation and invasion. Methods: Sixty-eight cases of patients with TSCC underwent radical surgery were selected from March 2013 to April 2015. The expressions of miR-30d in TSCC tissues and adjacent normal tissues were detected by using real-time fluorescent quantitative PCR. SCC-4 cells were cultured and were divided into ASO-miR-30d group, ASO-negative control group, and blank control group. The expression of miR-30d gene in each group was detected. The cell proliferation in each group was detected by MTT assay. The migration and invasion of cells in each group were detected by Transwell method. Results: The relative expression level of miR-30d in TSCC tissues was 1.89±0.21, which was higher than the adjacent normal tissues (1.31±0.16), and the difference was statistically significant (P=0.000). The relative expression level of miR-30d in TSCC tissues was correlated with TNM stage, differentiation, and cervical lymph node metastasis (P<0.05). Compared with the blank control group and the ASO-negative control group, the relative expression level of miR-30d in ASO-miR-30d group was significantly decreased (P<0.05). The results of MTT showed that the absorbance A values at 24 h, 48 h, 72 h, and 96 h in ASO-miR-30d group were significantly decreased (P<0.05). Compared with the blank control group and the ASO-negative control group, the number of migrating cells and the number of invasive cells in ASO-miR-30d group were decreased (P<0.05). Conclusion: miR-30d was highly expressed in TSCC tissues. Antisense miR-30d could effectively reduce TSCC cell proliferation and inhibit cell migration and invasion.

Key words: Tongue squamous cell carcinoma , miR-30d , Antisense oligodeoxynucleotides , Cell proliferation , Cell invasion

CLC Number:

R739.8

XU Xiao-fang, JIA Xiao-wei, LIANG Li-zhong.. Expression of miR-30d in Tongue Squamous Cell Carcinoma and Effect of Antisense miR-30d on Cell Proliferation and Invasion.[J]. Journal of Oral Science Research, 2017, 33(12): 1262-1265.

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