Effect of Porphyromonas Gingivalis Lipopolysaccharide (Pg-LPS) on the Expression of EphA2 Gene in Osteoclast.

Abstract

Abstract: Objective: To investigate the effect of porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the expression of EphA2 gene in RAW264.7 cells during osteoclastic differentiation. Methods: RAW264.7 cells were stimulated with 10μg/ml of porphyromonas gingivalis lipopolysaccharide (Pg-LPS) at 1, 3 and 5 d. RT-PCR was applied to determine the expression of EphA2 gene and the osteoclast related genes(MMP9,c-fos,ACP5,CtsK,NFATc1). Tartrate-resistant acid phosphatase (TRAP) staining was applied to observe osteoclast differentiation and maturation. Results: Compared with the control group, at the 3 d and 5 d, the EphA2 gene mRNA expression was significantly increased 2.4-fold and 1.2-fold in Pg-LPS group. At the 1 d, there was no obvious difference between the Pg-LPS group and control group. Meanwhile Pg-LPS stimulation significantly promote osteoclast related genes of c-fos, NFATc1, CtsK, ACP5 and MMP9 expressions.Tartrate-resistant acid phosphatase (TRAP) staining showed that compared with the control group, the number of the TRAP positive cells was significantly increased in the Pg-LPS group. Conclusion: Pg-LPS can promote the expression of EphA2 gene in the middle and later stages of osteoclast differentiation,but has no obvious effect in the early stage.

Key words: RAW264.7 , Pg-LPS, EphA2 gene

CLC Number:

R781.4

GAO Ai-chao, YU Hai-yan, LI Na, et al.. Effect of Porphyromonas Gingivalis Lipopolysaccharide (Pg-LPS) on the Expression of EphA2 Gene in Osteoclast.[J]. Journal of Oral Science Research, 2015, 31(3): 268-271.

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