Eexpression and Significance of Ptch and Smo in the Tongue Cancinoma Tca8113 Cell lines.

Abstract

Abstract: Objective: To investigate the expression and significance of the hedgehog signaling pathway factors Ptch and Smo in the tongue cancinoma Tca8113 cell lines. Methods: Aftter treatment of Tca8113 cells with hedgehog signaling pathway antagonist Cyclopamine, the proliferation-inhibition rate was detected by MTT assay. And mRNA and protein levels of Ptch and Smo were analyzed by RT-PCR and Western blot, respectively. Results: Cyclopamine inhibited Tca8113 cells. In the different times treatment of Tca8113 cells with Cyclopamine, the expression of Ptch and Smo mRNA in the Cyclopamine groups was lower than the control group, and the protein expression was the same with mRNA. In the 24h treatment of Tca8113 cells with Cyclopamine, the expression of Smo mRNA in the Cyclopamine groups was in dose-dependent manner, and in 72h the expression of Smo protein in the Cyclopamine groups was in dose-dependent manner. Conclusion: Cyclopamin specifically inhibited the Tca8113 cells migration and inhibited the expression of Smo mRNA and protein. The Hh signaling might be involved in the carcinogenesis and progression of tongue cancer.

Key words: Hedgehog signaling pathway, Tca8113 cells, Cyclopamine , Ptch, Smo

CLC Number:

R739.8

CHENG Zhi-fen, CUI Yan, XUAN Yan-hua.. Eexpression and Significance of Ptch and Smo in the Tongue Cancinoma Tca8113 Cell lines.[J]. Journal of Oral Science Research, 2015, 31(5): 433-436.

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