Journal of Oral Science Research

Biocompatibility of Pure Magnesium Ultrasonic Micro-arc Oxidation-HF-Silica Sol Compounded Treatment.

LIU Ji-guang, SUN Yu-long, LI Mu-qin, et al.

2015, 31(5): 429-432.

Abstract ( 138 )

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Objective: To study the biocompatibility of pure magnesium micro-arc oxidation-HF-silica sol composite treatment, and to evaluate the biocompatibility of pure magnesium micro-arc oxidation after different composite treatment. Methods: The experiment was divided into three groups, the pure magnesium micro-arc oxidation was used as the control group (group A). The pure magnesium micro-arc oxidation-silica sol composite treatment was group (group B). The pure magnesium micro-arc oxidation-HF-silica sol composite treatment was group (group C). MC3T3-E1 osteoblasts were inoculated on material surface of three groups, by scanning electron microscopy (SEM), laser confocal scanning microscope, CCK-8, ALP to test respectively growth, adhesion, proliferation and activity of osteoblasts. Results: The scanning electron microscope and laser confocal scanning microscope demonstrated that on cell growth and adhesion group B and group C surface were superior to group A. Group C was better than that of group B. The results of CCK-8 and ALP detection showed that the proliferation and activity of material surface of three groups were group C > group B > group A. The comparative differences of three groups were statistically significant. Conclusion: The pure magnesium micro-arc oxidation silicon sol composite treatment and pure magnesium micro-arc oxidation-HF-silicon sol composite treatment had all good biocompatibility, and the biocompatibility of HF-silica sol composite treatment was the best.

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Eexpression and Significance of Ptch and Smo in the Tongue Cancinoma Tca8113 Cell lines.

CHENG Zhi-fen, CUI Yan, XUAN Yan-hua.

2015, 31(5): 433-436.

Abstract ( 169 )

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Objective: To investigate the expression and significance of the hedgehog signaling pathway factors Ptch and Smo in the tongue cancinoma Tca8113 cell lines. Methods: Aftter treatment of Tca8113 cells with hedgehog signaling pathway antagonist Cyclopamine, the proliferation-inhibition rate was detected by MTT assay. And mRNA and protein levels of Ptch and Smo were analyzed by RT-PCR and Western blot, respectively. Results: Cyclopamine inhibited Tca8113 cells. In the different times treatment of Tca8113 cells with Cyclopamine, the expression of Ptch and Smo mRNA in the Cyclopamine groups was lower than the control group, and the protein expression was the same with mRNA. In the 24h treatment of Tca8113 cells with Cyclopamine, the expression of Smo mRNA in the Cyclopamine groups was in dose-dependent manner, and in 72h the expression of Smo protein in the Cyclopamine groups was in dose-dependent manner. Conclusion: Cyclopamin specifically inhibited the Tca8113 cells migration and inhibited the expression of Smo mRNA and protein. The Hh signaling might be involved in the carcinogenesis and progression of tongue cancer.

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3D Macroporous Nanofibrous Scaffolds of Gelatin Made by One-step Crosslink in Ethanol Bath.

DING Hui-fen, TENG Fang-jun, HAN Yu-ting Han, et al.

2015, 31(5): 437-440.

Abstract ( 191 )

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Objective: To prepare electrospinning nanofibrous scaffolds of water-soluble polymers with one-step crosslink technique in ethanol bath. Methods: The fibers received by the ethanol bath were prepared by the technique of one-step crosslink or two-step crosslink. The effect of different crosslink methods on the morphology, structure, crosslink degree, and stability of the fibers was analyzed. Results: Compared with the fibers prepared by the two-step crosslink, the gelatin fibers of one-step crosslink had thinner fiber diameters, larger pores, and higher water up-take, which could sustain the structure of the fibers and scaffolds. The degree of the crosslink could be changed by adjusting the concentration of the crosslinker and the time of crosslinking. The fibers of one-step crosslink were stable enough that additional crosslink treatment was not needed. Conclusion: The technique of one-step crosslink using ethanol bath could sustain the morphology and structure of the fibers, improve the efficiency of crosslink, and prepare the 3D macroporous nanofibrous scaffolds.

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An Experimental Study of Bio-oss with HIF-1α Protein on Tooth Extraction Bone Healing.

TAN Luan-jun, RUAN Zheng, ZHANG Jin-e, et al.

2015, 31(5): 441-443.

Abstract ( 170 )

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Objective: To study Bio-oss bone with HIF-1α protein on tooth extraction bone healing. Methods: Under general anesthesia, mesial root of the 2nd, 4th premolars in both sides of mandible of 3 Beagle dogs were extracted. Randomly, a wound was selected as the blank control group, and the rest were immediately implanted bio-oss, bio-oss with HIF-1α protein of 100ng/ml. After six and twelve weeks, the beagle dogs were sacrificed. The gross speciments were examined by Micro-CT images, and mineralized specimen preparations were prepared. Results: Micro-CT scan analysis showed that each group had some differences in the number of trabecular, bone mineral density and BV/TV. After twelve weeks,bio-oss with HIF-1α group was significantly higher than the bio-oss group、 the control group (P<0.05). The difference was statistical significance. Tissue slides also revealed an effective osteogensis in those wounds implanted with bio-oss with HIF-1α. Conclusion: Bio-oss with HIF-1α protein l can promote the regeneration of alveolar bone.

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Roles of Apoptosis and Mitosis in the Regeneration of Rat Parotid Gland following Ligation-induced Atrophy.

LU Hao, LIU Shi-wei, CUI Zhi, et al.

2015, 31(5): 444-447.

Abstract ( 143 )

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Objective: To investigate the roles of apoptosis and mitosis in the regeneration of rat parotid gland following release from duct ligation. Methods: The excretory duct was double ligated with metal-clip unilaterally near the hilum in order to induce atrophy of the parotid gland, and after 2 weeks of ligation(day 0)the double-ligated duct was reopened from 0 to 21 days in order to enable parotid gland regeneration. The evolving gland were examined with HE-staining, AB-PAS staining technique and immunohistochemistry for proliferating cell nuclear antigen(PCNA)as a marker of proliferating cells and Caspase3 as a marker of apoptotic cells. Results: After 14days ligation, the vast majority of acinus and the zymogen granules were replaced by the ductal structure. But during the regeneration of parotid gland, many residual and newly formed acinar cells were identified by HE and AB-PAS staining accompanying increasing zymogen granules. There were occasional PCNA-positive acinar or duct cells in ligated gland, but after 3d of the release of metal-clip, many PCNA-positive cells were seen especially acinar cells between 3d to 7d, thereafter PCNA-positive cells decreased in number.During the regeneration of parotid glands, the Caspase3-positive cells were very rarely observed in 0d, but Caspase3-positive numbers decreased after that. The difference of PCNA-positive numbers were statistically significant between each group (P<0.05)and the reaction of acinar cells compared to duct cells were significantly different at each point of the experimental group(P<0.05). Conclusion: During regeneration of the parotid gland, most acinar cells regeneration from the residual acinar cells at the peripheral region of lobules and the apoptosis of did not play important roles in the regeneration of rat parotid gland after 2 weeks duct-ligation.

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Expression of Wnt3a and DKK1 during Orthodontic Tooth Movement.

LU Juan, DUAN Ying-ying, WU Ming-ming, et al.

2015, 31(5): 448-450.

Abstract ( 200 )

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Objective: To observe the changes of Wnt3a and DKK1 expressions in periodontal tissue during orthodontic tooth movement (OTM). Methods: Thirty male SD rats were randomly divided into six groups according to tooth movement time points of 1d, 3d, 5d, 7d, 10d, 14d. Nickel-titanium closed-coil springs were used to deliver an initial 50 g mesial force to the left maxillary first molars. The right maxillary molars without force application served as control. Immunohistochemical staining technology was used to detect the expression of Wnt3a and DKK1. Results: Wnt3a and DKK1were expressed on both the ipsilateral and contralateral sides in each group. On the tension side, expression of Wnt3a and DKK1 elevated firstly and then decreased, peaked at day 5 and day 7 respectively. On the compression side, Wnt3a expression decreased fisrtly and then increased while DKK1 quite the opposite, peaked at day 5 and day 10 respectively. Conclusion: Wnt3a and DKK1 participated in periodontal tissue remodeling, suggesting that Wnt signaling pathway may be one of regulation pathways in periodontal tissue remodeling during OTM.

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Expression of Connective Tissue Growth Factor in Rat Mandibular Condylar Cartilage Following Function Mandibular Forward Positioning.

YU Ying, AI Hong, TU Shao-qin, et al.

2015, 31(5): 451-456.

Abstract ( 162 )

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Objective: To investigate the expression of connective tissue growth factor (CTGF) in rat mandibular condylar cartilage during functional mandibular forward positioning with immunohistochemical staining. Methods: 45 five-week-old female Sprague-Dawley (SD) rats were randomly divided into five experimental groups (n=25) and five control groups (n=20). The animals in the experimental groups were fitted with modified removable acrylic inclined planes to advance the mandible, while rats in the normal control groups were left intact. The rats were sacrificed on day 3, 7, 14, 21, and 30, respectively, to assess the expression of CTGF with immunohistochemical staining. Results: When compared with the proliferative zone of the same age in the control and experimental groups, higher expression of CTGF was observed in the hypertrophic zone of posterior part of condylar cartilage (P<0.05) . The expression of CTGF in the proliferative zone of posterior part of condylar cartilage in the control groups did not show significant differences during the experiment period, and the hypertrophic zone displayed the same pattern. However, compared with those of the age-matched controls, the positive signals of CTGF in the proliferative zone and hypertrophic zone of the experimental animals were significantly stronger on day 7, 14, and 21, respectively (P<0.05), whereas no statistical differences were found on days 3 and 30. Conclusion: CTGF expression in mandibular condylar cartilage is induced by the mechanical compressive stimuli in vivo. As a cytokine, CTGF may mediate the morphological changes induced by mechanical stimuli in condylar cartilage.

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In Vitro Studies on the Cytotoxicity of Three Commercial Denture Adhesives.

CHEN Feng-ying, CHENG Xiang-rong.

2015, 31(5): 457-460.

Abstract ( 146 )

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Objective: To investigate the cytotoxicity of three commercial denture adhesives. Methods: The cytotoxic effects of three denture adhesive eluates with different concentrations on primary human oral keratinocytes(HOKs), fibroblasts (HOFs) and permanent mouse fibroblasts cell lines (L929) were tested using MTT assay. Results: Three tested denture adhesives showed cytotoxicity to primary HOKs(levels 1 to 2) and HOFs( level 1), whereas none of three was toxic to L929 cells. Higher concentrations of adhesive eluates generally showed significantly stronger inhibitory effects than the lower ones. Conclusion: Denture adhesives are toxic to the primary HOKs and HOFs cultures, whereas non-toxic to L929 cells. The results suggest that primary human oral mucosal cells may provide more valuable information in toxicity screening of denture adhesives.

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Study of Caries Microbial Community Structure and Diversity by 16S rRNA High-throughput Sequencing.

WANG Shao-guo, YOU Xiang-lei, CHE Chun-xiao, et al.

2015, 31(5): 461-465.

Abstract ( 255 )

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Objective: To study the oral microbial community structure in caries-active and caries-free objects using 16S rRNA high-throughput sequencing. Methods: 14 volunteers in Kangle County, Gansu Province were selected, 7 caries- active cases (DMFT≥4, CA group), 7 caries-free cases (CF group), saliva DNA were extracted and PCR amplification. Using Illumina Miseq sequencing platform, two-terminal sequencing of V4 areas of 16S rRNA was carried; diversity of community structure was analyzed using the software such as Mothur, MEGAN4, Cluster 3.0 and Java Treeview software. Results: In total, 118151 measured sequences, 5738 OTUs and 2795 species were found in 10 samples, belonging to 27 phyla, 218 genera. There were some discrepancies between CA group and CF group, at the phylum level, Firmicutes and SR1 in CA group were lower than in CF group. At the genus level, Rothia, Alistipes and Catonella in CA group were lower than in CF group. Scardovia in CA group was higher than in CF group;OTUs were 550.2±26.48 in CA group and 597.4±66.07 in CF group, Simpson index was 0.73±0.03 in CA group and 0.49±0.01 in CF group, but both of them had no statistical difference between the two groups. Conclusion: The microbial community structure in saliva were complicated; the variety in CA group was lower than that in CF group; The existence of Rothia, Alistipes and Catonella et al. had a negative correlation with caries, otherwise Scardovia et al. had a positive correlation with caries.

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Vitamin C Enhanced the Expressions of Histone Demethylase KDM2B and KDM5C in Stem Cells from Apical Papilla.

WANG Yue-jun, LIU Da-yong, FAN Zhi-peng, et al.

2015, 31(5): 466-469.

Abstract ( 166 )

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Objective: To investigate whether Vitamin C could regulate the expression of histone demethylases in stem cells from apical papilla (SCAPs). Methods: Different doses of Vitamin C were used to stimulate the SCAPs. The qRT-PCR was used to detect the expression of histone demethylases at the mRNA level. Results: The qRT-PCR results showed that the expressions of KDM2B and KDM5C were up-regulated at 2, 4 and 8h after 10 μmol/L Vitamin C treatment in a time dependent manner. And 5, 10 and 20 μmol/L Vitamin C could increase the expressions of KDM2B and KDM5C in a dose dependent manner at 8h. Conclusion: Vitamin C could induce the expressions of histone demethylase KDM2B and KDM5C in SCAPs.

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Study on the Perioral Soft Tissue Changes with Age for Normal Occlusion Subjects.

QI Wei-ting, LIU Chun-yan, WANG Wen, et al.

2015, 31(5): 470-474.

Abstract ( 175 )

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Objective: To investigate the changes of the perioral soft tissue in subjects at rest position and posed smile. Methods: 240 subjects with normal occlusion at the age of 15-49 were selected. The images were recorded and the perioral soft tissue were measured with the software of Smile Measure. Results: The analysis of pairwise comparison showed there were significant differences in the upper lip length at rest, upper lip length at smile, upper lip thickness at rest, upper lip thickness at smile, intercommissural width at rest, change in intercommissural width, maxillary incisal display, interlabial gap, smile index, and buccal corridors between each two groups. The analysis of two-sample t test showed there were significant differences in upper lip length at rest, upper lip length at smile, change in upper lip length, intercommissural width at rest between the male and female in the same group. The analysis of chisquare test showed there were significant differences between the smile height of male and female in G1,G2,G3,G4. Conclusion: With age increasing, upper lip length increased, the lip became more thinner, intercommissural became more wider and buccal corridors became more larger, and smile tended to be low smile. There were gender differences in lips and teeth relationship of subjects at the same age.

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Early Loading at the 3rd Week of SLActive Implant in the Posterior Mandible: 1-Year Results of a Clinical Observation.

GENG Wei, LIN Xiao, LI Xiao-guang, et al.

2015, 31(5): 475-478.

Abstract ( 168 )

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Objective: To investigate the feasibility of SLActive implants which were functionally loaded after 3 weeks of healing. Methods: 32 partially edentulous patients had a total of 40 implants inserted in the posterior mandibles and underwent undisturbed healing for 3 weeks. The implants were loaded with provisional crowns in the 3rd week. Resonance frequency analysis was assessed by using Osstell MentorTM in the procedure (baseline) and after 1, 3, 12 weeks and 1 year of implants placement. The X-ray cone paralleling radiographs were obtained at 1,3, 12 week and 1 year as well. Results: Statistic analysis revealed that there was a significant difference (P<0.01) between the 3-week and 12-week ISQ values of the implants loaded at the 3rd week, as well as 3-week and 1-year values. The marginal bone was absorbed 0.5±0.21mm within a year after the implants inserted. All the implants were stable without peri-implantitis. The success rate was 100%. Conclusion: The study of using 3-week early loading protocol demonstrates that SLActive implants inserted in posterior mandible may achieve prospective outcomes over a period of 1 year.

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Systematic Review and Meta Analysis of Levamisole or Combined with Traditional Chinese Medicine in the Treatment of Recurrent Aphthous Ulcer.

ZHANG Yi-fan, QIN Xuan, LIN Juan, et al.

2015, 31(5): 479-484.

Abstract ( 161 )

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Objective: To evaluate the effectivness and safety of levamisole in the treatment of recurrent aphthous ulcer. Methods: The databases such as Cochrane Library, PubMed, CNKI and Wanfang Data were searched by computer to include randomized controlled trails(RCTs) on levamisole in the treatment of recurrent aphthous ulcer. Studies were screened according to the inclusion and exclusion criterion. Data were extracted and the meta-analyses were performed by using RevMan 5.2 software. Results: 9 studies(331 patients) were included in the review of levamisole in treatment of RAU. The results of meta-analyses showed, compared with placebo group, levamisole was superior in improving RAU(OR=4.92,95%CI[2.14,11.32],P=0.0002). 46 patients were reported adverse reactions. 16 studies(1854 patients) were included in the review of levamisole combined with traditional chinese medicine in treatment of RAU. The results of meta-analyses showed, compared with placebo group, levamisole was superior in improving RAU(OR=4.92,95%CI[2.14,11.32],P=0.0002). No one were reported with adverse reactions. Conclusion: Current evidences show that levamisole combined with TCM is likely to be more effective and safety in the treatment of RAU than using levamisole only. However, for the limited evidence and lower methodological quality of the included studies, this conclusion still needs to be verified with more high-quality RCTs.

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Study of Apical Surface Morphology after Root Canal Preparation with Different Kinds of Nickel-titanium Instruments Horizontal to the Anatomic Apical.

LIU Zong-xiang, LIU Yi, DUAN Jun-lan, et al.

2015, 31(5): 485-489.

Abstract ( 178 )

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Objective: To study the apical surface morphology after root canal preparation with different nickel-titanium instruments horizontal to the anatomic apical. Methods: 40 freshly extracted tooth due to periodontal disease which were randomized to 2 groups were taken images by SEM to record the apical surface morphology. Then they were prepared with K3 or TF respectively under Work Length (horizontal to the anatomical apical foramen). After root preparation, the apical surface morphology of each tooth was observed by SEM, then the apical foramen shift, dentin defects and crackle surrounding the apical foramen was recorded. Results: The apical foramen diversifications (include apical foramen shift, dentin defects and crackle) were observed in both K3 group and TF group. The difference of apical foramen shift and crackle number between two groups didn’t have statistically significant while the numbers of tooth which had dentin defects between two groups have statistically significant(P<0.05). Conclusion: The integrity of the apical foramen would be destroyed after root canal preparation with two kinds of nickel-titanium instruments horizontal to the anatomic apical maybe due to super the apical foramen. The surface morphology of apical foramen would have a lot of diversifications include apical foramen shift, dentin defects and crackle cracks and dentin defects surrounding the apical. Compare with k3 file, TF file group have less apical foramen shift and dentin defects.

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Retrospective Study for Etiologies and Treatments of Odontogenic Maxillary Sinusitis.

MENG Tian, ZHANG Zhi-yong.

2015, 31(5): 490-492.

Abstract ( 199 )

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Objective: To analyze the affected tooth, the common causes, the treatments and the outcomes of odontogenic maxillary sinusitis. Methods: Cases about patients affected by odontogenic maxillary sinusitis in First Clinical Division of Peking University School and Hospital of Stomatology from January 2000 to December 2012 were collected and retrospectively analyzed. Results: 23 cases were collected. The diseases of maxillary first molars caused 47.7% of odontogenic maxillary sinusitis cases. Chronic periapical periodontitis caused 78.3% of odontogenic maxillary sinusitis cases. The treatments should combine treating dental diseases, medicine and sinus surgeries. The outcomes were satisfactory. Conclusion: Diseases of maxillary first molars were the most common to cause odontogenic maxillary sinusitis. Most of odontogenic maxillary sinusitis were caused by chronic periapical periodontitis. Combined treatments could get good results.

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Preliminary Study on Identification of Drug Resistant and Non-drug Resistant Oral Squamous Carcinoma Cell line Tca8113 Using Metabolomics.

WANG Hui, Meng Ya, ZHANG Ping.

2015, 31(5): 493-496.

Abstract ( 143 )

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Objective: To identify the drug resistant and non-drug resistant of oral squamous carcinoma Tca8113. Methods: Cell growth curve was drafted and drug sensitivity were detected by the MTT assay. The immunohistochemistry was applied to detect the expression of P-gp which was related to multidrug resistance phenomenon in drug resistant cells. Extracellular metabolites of all cell lines was collected and was detected by nuclear magnetic resonance (NMR). Data analysis used principal component analysis(PCA). Results: The principal component analysis showed the obvious clustering relations in the data of drug resistant and non-drug resistant cells, indicating 1H NMR-based metabolomics method could distinguish between drug resistant and non-drug resistant Tca8113. Conclusion: The metabolomics is expected to be favorable application prospect in rapid identification of oral cancer cells with resistance, and it is expected to become a new testing method in future.

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Dental Cone Beam Computed Tomography Analyses of Resorption of Labial Bone in Maxillary Anterior Implant by Bone Splitting Technique.

WANG Wen-jun, MA Min.

2015, 31(5): 497-499.

Abstract ( 176 )

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Objective: To evaluate the vertical bone resorption of labial bone in anterior maxillary implant by bone splitting technique. Methods: 19 patients (8 men, 11 women) whose ages ranged from 22 to 65 years, were included in this study. The labial-lingual thickness of alveolar was from 3.0 to 5.0mm for everybody, 26 Ankylos implants (3.5mm)were placed after splitting the bone immediately and constructed with the operation of guided bone regeneration, CBCT were taken after crown setted immediately and two years later to observe the labial bone resorption. The value of the labial bone resorption and gingival recession were calculated and analysed and the correlation between these changes were detected in SPSS 11.0. Results: Both vertical bone resorption and gingival recession showed a significant negative correlation to cervical width. As expected, vertical bone resorption and gingival recession showed a significant positive correlation. Conclusion: It is suggested that alveolar bone thickness after implant placement in the anterior region could be negatively associated with the level of alveolar bone resorption at the labial aspect as well as gingival recession.

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Advanced Glycation End Products Effect on the Proliferation of Human Periodontal Ligament Stem Cells and Its Effect on HSG and Cyclin D1 Expression.

TAO Tin-liang, DENG Chao, NIU Hai, et al.

2015, 31(5): 500-502.

Abstract ( 172 )

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Objective: To investigate the effect of advanced glycation end products ( AGEs) on the proliferation of human periodontal ligament stem cells ( HPDLSCs). Methods: HPDLSCs were isolated by limited dilution of culture cells for single cell clone. The osteogenic differentiation capacity of HPDLSCs was evaluated by Alizarin red staining. The adipogenic differentiation capacity of HPDLSCs was evaluated by oil red staining. HPDLSCs were induced with different concentrations of AGEs, The proliferation of HPDLSCs was assayed by MTT, Real time quantitative reverse transcription polymerase chain reaction (real time PCR) was performed to detect the differences of gene expression between the control group and experimental group. Results: After 21 days induction,Alizarin red staining showed mineralization nodules were formed, oil red staining showed lipid droplets were formed. Different concentrations of AGEs had different effects on the PDLSCs proliferative capacity. High concentrations (100mg/L, 200mg/L) significantly inhibited the proliferation of PDLSCs. Low concentration (1mg/L, 10mg/L) had little effect on the proliferative capacity of PDLSCs. After 3 days, the expressions of cell cycle gene (cyclinD1) in the experimental group were lower than those in the control group, the expressions of HSG in the experimental group were higher than those in the control group (P<0.05). Conclusion: High concentrations of AGEs reduced the proliferation capacity of HPDLSCs,and changed the expressions of HSG and cyclinD1 mRNA levels.

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Relationship between Methods of Extract Mandibular Third Tooth and TMD.

LIU Zhi-ming, ZHANG Zhou-wen, HUANG Li, et al.

2015, 31(5): 503-502.

Abstract ( 185 )

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Objective: To investigate the relationship between methods of extract tooth and TMD. Methods: 160 individual were randomly chosed. Minimally invasive and traditional extract tooth methods were used respectively to extract impacted tooth of third molar of mandibular. Dual-axis diagnosis standard was used to analyse the symptom of TMD after operation of extract the tooth in pre-operation, post-operation 1, 4, 24 weeks. Results: The group of minimally invasive morbidity of temporomandibular joint click was significantly lower than the traditional extract tooth methods group, and this difference was statistically significant. Conclusion: Minimally invasive extract tooth methods can reduce the complication of TMD.

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Observation of the Ultrastructural Changes of Sclerotic Dentin Pretreated with EDTA by SEM.

LI Ting-ting, SUN Min-min, KANG Jun-tang, et al.

2015, 31(5): 506-507.

Abstract ( 166 )

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Objective: To evaluate the ultrastructure changes of sclerotic dentin in non-carious cervical lesions before and after pretreated with EDTA(Ethylene Diamine Tetraacetic Acid). Methods: 10 teeth with scale Ⅲ or Ⅳ sclerotic dentin were selected. The ultrastructural changes of them before and after pretreated with EDTA were observed by SEM. Rusults: After pretreated the sclerotic dentin by EDTA, the top dentinal tubules of the calcium salt deposits were completely dissolved, and there still has residual dissolved calcium salt particle in the deep dentinal tubules. Conclusion: EDTA could improve the poor adhesion of the sclerotic dentin surface and increase the bond strength.

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Clinical Curative Effect Observation of Trigeminal Neuralgia Patients Treated with Selective Terminal Branch Radiofrequency Therapy.

YU Huai-zhou, YAO Shi-hong, ZHAO Su-li , et al.

2015, 31(5): 508-510.

Abstract ( 148 )

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Objective: To study the clinical curative effect of treating trigeminal neuralgia patients with selective terminal branch radiofrequency therapy. Methods: 158 cases of primary trigeminal neuralgia patients were chosen and treated with RF (radiofrequency) therapy by determining the dominant Terminal branches according to the attack range. Results: 156 cases respond effectively to the first RF treatment and the effective rate reaches 98.73%. 42 among them have a relapse afterwards but respond well to the second RF treatment. None of these cases suffered from serious complications. Conclusion: It is quite safe to treat trigeminal neuralgia patients with selective terminal branch radiofrequency therapy and the curative effects tend to be satisfactory. As a minimally invasive method, the selective terminal branch radiofrequency therapy proves to have great clinical practical value.

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Evaluation of Apical Sealing Ability of β-Ca₂SiO₃/CSH Composite Materials in Vitro.

ZHAO Yu, JIA Hong-yu, LIU Ming.

2015, 31(5): 511-512.

Abstract ( 139 )

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Objective: To observe the apical sealing ability of β-Ca₂SiO₃/CSH composite materials. Methods: Sixty-six premolars with the sigle root canal were used in this study. The teeth were randomly divided into six test groups,10 in each group. A was C_1.4Si, B was C_1.4Si/CSH₁₀, C was C_1.4Si/CSH₃₀, D was C_2.0Si, E was C_2.0Si/CSH₁₀, F was C_2.0Si/CSH₃₀. In addition,there were positive and negative control groups, 3 in each group. Protaper was used to prepare the root canal and the composite was filled into the root canal. The method of the dye penetration was performed to evaluate the apical sealing ability. Results: There were significant differences of the apical sealing ability between A and C or D and F(P<0.05). Conclusion: The more CSH of β-Ca₂SiO₃/CSH complex was, the larger value of the apical microleakage was.

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Survey and Analysis of Knowledge on Oral Mucous Diseases among College Students.

LI Hong, YANG xiao-wen, SHI Jing, et al.

2015, 31(5): 513-516.

Abstract ( 135 )

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Objective: To investigate the knowledge on oral mucous diseases among college students. Methods: A survey with a self-designed questionnaire was conducted among 331 college students. Furthermore, the data were statistically analyzed. Results: The phenomena of smoking, drinking and unhealthy diet were widespread in these students. 81.87% of the students had never contacted the knowledge of oral mucous diseases in any way. During various approaches, health education from school and the dentists was account for minimum proportion. The awareness rates of all kinds of knowledge on oral mucous diseases were fairly low. Conclusion: The lack of knowledge on oral mucous diseases is quite heavily in the students. The schools and the dentists should cooperate and work actively to promote the health education of oral mucous diseases among college students.

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Influence of Compliance of Patients with Chronic Periodontitis on Clinical Effect during Periodontal Maintenance.

GUANG Tian-yan.

2015, 31(5): 517-519.

Abstract ( 138 )

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Objective: To observe the different influence of compliance of patients with chronic periodontitis on clinical effect during periodontal maintenance. Methods: 70 cases of patients with chronic periodontitis were selected in our hospital. According to patient compliance, they were divided into complete compliance group and incomplete compliance group, 35 cases in each group. All patients were given the foundation treatment for 4 weeks and patients without operation indication were given periodontal maintenance therapy and regular reexamination.Before treatment and after 12 months of treatment in patients with plaque index (Plaque, index, PI), gingival index (Gingival, index, GI) and the depth of periodontal pocket (Probing depth, PD) and other indicators. Results: Before treatment, PI, GI and PD of two groups of patients had no significant difference. After 12 months of treatment, PI, GI and PD of the complete compliance group were (0.23±0.14), (0.84±0.23) and (0.23±0.14), PI, GI and PD of incomplete compliance group were (0.81±0.19), (1.12±0.30) and (0.81±0.19), (P<0.05). Conclusion: Better compliance of the chronic periodontitis patients had better curative effect than the poor compliance of patients.

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Effect of Ozonized Water on Dentin Micro Hardness of Human Teeth.

ZHU Di, YU Hai-li, LIU Li-mei.

2015, 31(5): 520-521.

Abstract ( 157 )

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Objective: To study the effect of ozonized water as endodontic irrigation solvents on dentin micro-hardness of human teeth. Methods: Extracted human premolar crowns were cut along long axis and treated with 2.15mg/L ozonized water, 1%NaOCL, 3%H2O2, 15%EDTA and distilled water respectively for 5min and 15min. The micro hardness of dentin was measured. Results: 1%NaOCL, 3%H₂O₂ and 15%EDTA decreased the micro hardness of dentin, especially 15%EDTA, while ozonized water was harmless to dentin micro hardness. Conclusion: It is recommended to apply 2.15mg/L ozonized water as endodontic irrigation.

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