Abstract: Objective: To study the biocompatibility of pure magnesium micro-arc oxidation-HF-silica sol composite treatment, and to evaluate the biocompatibility of pure magnesium micro-arc oxidation after different composite treatment. Methods: The experiment was divided into three groups, the pure magnesium micro-arc oxidation was used as the control group (group A). The pure magnesium micro-arc oxidation-silica sol composite treatment was group (group B). The pure magnesium micro-arc oxidation-HF-silica sol composite treatment was group (group C). MC3T3-E1 osteoblasts were inoculated on material surface of three groups, by scanning electron microscopy (SEM), laser confocal scanning microscope, CCK-8, ALP to test respectively growth, adhesion, proliferation and activity of osteoblasts. Results: The scanning electron microscope and laser confocal scanning microscope demonstrated that on cell growth and adhesion group B and group C surface were superior to group A. Group C was better than that of group B. The results of CCK-8 and ALP detection showed that the proliferation and activity of material surface of three groups were group C > group B > group A. The comparative differences of three groups were statistically significant. Conclusion: The pure magnesium micro-arc oxidation silicon sol composite treatment and pure magnesium micro-arc oxidation-HF-silicon sol composite treatment had all good biocompatibility, and the biocompatibility of HF-silica sol composite treatment was the best.

Key words: Pure magnesium , Micro-arc oxidation, Hydrogen fluoride , Silica sol, Osteoblast