Biocompatibility of Lactoferrin Composite Membrane on Pure Titanium Surface Treated by Micro-arc Oxidation Technology

doi:10.13701/j.cnki.kqyxyj.2019.06.020

Abstract

Abstract: Objective: To investigate the effect of microarc oxidation of titanium alloy with silane coupling agent manned and bovine lactoferrin on bone binding ability. Methods: Titanium alloy microarc oxidized (MAO) silane was used as control group (A). Experiment groups were: titanium alloy-MAO-silane-bovine lactoferrin (BLF) group (B) and titanium alloy-MAO-silane-human lactoferrin (HLF) group (C). They were implanted into the mandible of rabbits at random. Through cone beam three-dimensional imaging equipment (CBCT), the bonding between titanium implant and bone tissue was observed. And through scanning electron microscopy, the thickness of new bone and the morphology of implant-bone interface after implantation were observed. Results: Through CBCT, with the increase of time, the bone density around the implant increased and the range of transmission image narrowed. Group (C) was better than group (B), and group B was better than group A. After 8 weeks, the bone tissue healing was better in group (C), and group (B) was better than group (A). Scanning electron microscopy showed that the composite membranes in group (C) distributed evenly on the implant surface, and compared with group (A) and (B), the contents of Ca and P increased, the new bone quantity increased, and the elements of the composite membranes penetrated into the surrounding bone tissue more extensively. Conclusion: Titanium alloy microarc oxidation silane coupled with human lactoferrin composite membrane could shorten the time of bone binding and might have more osteoinductive ability.

Key words: Ttitanium alloy, Micro-arc oxidation, Silane coupling agent, lactoferrin synostosis

ZHANG Yi-dan,XIAO Yue,LI Mu-qin,YAO Hai-tao,YUE Shen-shen,ZHENG Yu-jing. Biocompatibility of Lactoferrin Composite Membrane on Pure Titanium Surface Treated by Micro-arc Oxidation Technology[J]. Journal of Oral Science Research, 2019, 35(6): 595-599.

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