Advanced Glycation End Products Effect on the Proliferation of Human Periodontal Ligament Stem Cells and Its Effect on HSG and Cyclin D1 Expression.

Abstract

Abstract: Objective: To investigate the effect of advanced glycation end products ( AGEs) on the proliferation of human periodontal ligament stem cells ( HPDLSCs). Methods: HPDLSCs were isolated by limited dilution of culture cells for single cell clone. The osteogenic differentiation capacity of HPDLSCs was evaluated by Alizarin red staining. The adipogenic differentiation capacity of HPDLSCs was evaluated by oil red staining. HPDLSCs were induced with different concentrations of AGEs, The proliferation of HPDLSCs was assayed by MTT, Real time quantitative reverse transcription polymerase chain reaction (real time PCR) was performed to detect the differences of gene expression between the control group and experimental group. Results: After 21 days induction,Alizarin red staining showed mineralization nodules were formed, oil red staining showed lipid droplets were formed. Different concentrations of AGEs had different effects on the PDLSCs proliferative capacity. High concentrations (100mg/L, 200mg/L) significantly inhibited the proliferation of PDLSCs. Low concentration (1mg/L, 10mg/L) had little effect on the proliferative capacity of PDLSCs. After 3 days, the expressions of cell cycle gene (cyclinD1) in the experimental group were lower than those in the control group, the expressions of HSG in the experimental group were higher than those in the control group (P<0.05). Conclusion: High concentrations of AGEs reduced the proliferation capacity of HPDLSCs,and changed the expressions of HSG and cyclinD1 mRNA levels.

Key words: Advanced glycation end products , Human periodontal ligament stem cells , Proliferation

CLC Number:

R781.4

TAO Tin-liang, DENG Chao, NIU Hai, et al.. Advanced Glycation End Products Effect on the Proliferation of Human Periodontal Ligament Stem Cells and Its Effect on HSG and Cyclin D1 Expression.[J]. Journal of Oral Science Research, 2015, 31(5): 500-502.

References

[1] M.Brownlee H, Vlassara A, Kooney, et al. Aminoguanidine prevents diabete-induced arterial wall protein cross-linking [J]. Science, 1986, 232(4758)∶1629-1632
[2] Enomoto M, Adachi H, Yamagishi S, et al. Positive association of serum levels of advanced glycation end products with thrombogenic makers in humans [J]. Metabolism, 2006, 55∶912-917
[3] Loe H, Periodontal disease. The sixth complication of diabetes mellitus [J]. Diabetes Care, 1993, 16(1)∶329-334
[4] Covani U, Marconcini S, Derchi G, et al. Relationship between human periodontitis and type2 diabetes at a genomic level:a data-mining study [J]. J Periodontol,2009,80(8)∶1265-1273
[5] Wu X-D, Liu W-L, Zeng K, et al. Advanced glycation end products activate the miRNA/RhoA/ROCK2 pathway in endothelial cells [J]. Microcirculation, 2014, 21∶178-186
[6] Morita M,Yano S,Yamaguchi T, et al. Advanced glycation end products induced reactive oxygen species generation is partly through NF-kappa B activation in human aortic endothelial cells [J]. J Diabetes Complications, 2013, 27∶11-15
[7] Kang R, Tang D, Schapiro NE, et al. The receptor for advanced glycation end products (RAGE) sustains autophagy and limits apoptosis, promoting pancreatic tumor cell survival [J]. Cell Death Differ, 2010, 17∶666-676
[8] Sadik NA, Mohamed WA, Ahmed MI. The association of receptor of advanced glycated end products and inflammatory mediators contributes to endothelial dysfunction in a prospective study of acute kidney injury patients with sepsis [J]. Mol Cell Biochem, 2012, 359∶73-81
[9] Santel A,Fuller MT,Control of mitochondrial morphology by a human mitofusin [J]. J Cell Sci,2001,114(5)∶867-874
[10] Chen KH, Guo XM, Dalong MA, et al. Dysregulation of HSG triggers vascular proliferative disorders [J]. Nature Cell Biol,2004,6(9)∶872-883
[11] Zhang Y, DU Q, Qiu XY, et al. Over expression of hyperplasia suppressor gene inhibits the malignant phenotype of breast cancer cell [J]. Zhonghua Bing Li Xue Za Zhi, 2010, 39(4)∶259-263

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