Effects of Cinnamaldehyde on Proliferation and Differentiation of MC3T3-E1 Osteoblasts Induced by High Glucose

doi:10.13701/j.cnki.kqyxyj.2019.07.022

Abstract

Abstract: Objective: To observe the effects of cinnamaldehyde (CA) on the proliferation and differentiation of MC3T3-E1 osteoblasts induced by high glucose. Methods: MC3T3-E1 cells were cultured in glucose (25 mmol/L) with 0 (control), 10, 20, and 40 mg/L CA. Cell proliferation, ALP activity, and alizarin red staining was measured. Morphology of the cytoskeleton was observed by confocal laser scanning microscope. Expression levels of osteogenesis related genes OCN mRNA were detected by real-time PCR. Results: CA could promote the proliferation and ALP activity of osteoblasts in high glucose environment (P<0.05). The number and the volume of mineralized nodules increased. The expression of OCN mRNA in the experimental group was significantly up-regulated (P<0.05). When the concentration of CA was 10mg/L and 20mg/L, the cell spreading area was increased and the cytoskeleton was clearer. Conclusion: CA can promote the proliferation and differentiation of MC3T3-E1.

Key words: Cinnamaldehyde, MC3T3-E1 , OCN, Proliferation

WANG Songsong, MA Xiangrui, ZHANG Yuntao. Effects of Cinnamaldehyde on Proliferation and Differentiation of MC3T3-E1 Osteoblasts Induced by High Glucose[J]. Journal of Oral Science Research, 2019, 35(7): 712-715.

References

[1] Dakovic D, Pavlovic MD. Periodontal disease in children and adolescents with type 1 diabetes in Serbia [J]. J Periodontol, 2008, 79 (6) :987-992.
[2] Oates TW, Huynh-Ba G, Vargas A, et al. A critical review of diabetes, glycemic control, and dental implant therapy [J]. Clin Oral Impl Res, 2013, 24 (2) :117-127.
[3] Zhu R, Liu H, Liu C, et al. Cinnamaldehyde indiabetes: a review of pharmacology, pharmacokineticsand safety [J]. Pharmacol Res, 2017, 122:78-89.
[4] Wu Z, Weng S, Yan D, et al. Administration of cinnamaldehyde promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro [J]. J Pharmacol Sci, 2018, 138(1):63-70.
[5] 苏野,邵聆.1型糖尿病小鼠下颌密质骨厚度特点的研究[J].口腔医学研究,2018,34(6):619-622.
[6] Beejmohun V , Peytavy-Izard M , Mignon C , et al. Acute effect of Ceylon cinnamon extract on postprandial glycemia: alpha-amylase inhibition, starch tolerance test in rats, and randomized crossover clinical trial in healthy volunteers [J]. BMC Complementary Altern Med, 2014, 14(1):351.
[7] Zhu R, Liu H, Liu C, et al. Cinnamaldehyde in diabetes: A review of pharmacology, pharmacokinetics and safety [J]. Pharmacol Res, 2017,122:78-89.
[8] Wu Z, Yan D, Xie Z, et al. Combined treatment with cinnamaldehyde and PTH enhances the therapeutic effect on glucocorticoid-induced osteoporosis through inhibiting osteoclastogenesis and promoting osteoblastogenesis [J]. Biochem Biophys Res Commun, 2018, 505(3):945-950.
[9] Martin M, Valencia K, Zandueta C, et al. The usefulness of bone biomarkers for monitoring treatment disease:a comparative study in osteolytic and osteosclerotic bone metastasis models [J]. Translational Oncology, 2017, 10(2) :255-261.
[10] Ferron M, Hinoi E, Karsenty G, et al. Osteocalcin differentially regulates beta cell and adipocyte gene expression and affects the development of metabolic diseases in wild-type mice [J]. Proc Natl Acad Sci U S A, 2008, 105 (13) :5266-5270.
[11] Jones TJ, Adapala RK, Geldenhuys WJ, et al. Primary cilia regulates the directional migration and barrier integrity of endothelial cells through the modulation of hsp27 dependent actin cytoskeletal organization [J]. J Cell Physiol, 2012, 227(1):70-76.

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