Abstract: Objective: To identify the drug resistant and non-drug resistant of oral squamous carcinoma Tca8113. Methods: Cell growth curve was drafted and drug sensitivity were detected by the MTT assay. The immunohistochemistry was applied to detect the expression of P-gp which was related to multidrug resistance phenomenon in drug resistant cells. Extracellular metabolites of all cell lines was collected and was detected by nuclear magnetic resonance (NMR). Data analysis used principal component analysis(PCA). Results: The principal component analysis showed the obvious clustering relations in the data of drug resistant and non-drug resistant cells, indicating 1H NMR-based metabolomics method could distinguish between drug resistant and non-drug resistant Tca8113. Conclusion: The metabolomics is expected to be favorable application prospect in rapid identification of oral cancer cells with resistance, and it is expected to become a new testing method in future.

Key words: Oral squamous carcinoma , Multidrug resistance(MDR) , Metabolomics, Nuclear magneticresonance(NMR) , Principal components analysis(PCA)