Expression of Wnt3a and DKK1 during Orthodontic Tooth Movement.

Abstract

Abstract: Objective: To observe the changes of Wnt3a and DKK1 expressions in periodontal tissue during orthodontic tooth movement (OTM). Methods: Thirty male SD rats were randomly divided into six groups according to tooth movement time points of 1d, 3d, 5d, 7d, 10d, 14d. Nickel-titanium closed-coil springs were used to deliver an initial 50 g mesial force to the left maxillary first molars. The right maxillary molars without force application served as control. Immunohistochemical staining technology was used to detect the expression of Wnt3a and DKK1. Results: Wnt3a and DKK1were expressed on both the ipsilateral and contralateral sides in each group. On the tension side, expression of Wnt3a and DKK1 elevated firstly and then decreased, peaked at day 5 and day 7 respectively. On the compression side, Wnt3a expression decreased fisrtly and then increased while DKK1 quite the opposite, peaked at day 5 and day 10 respectively. Conclusion: Wnt3a and DKK1 participated in periodontal tissue remodeling, suggesting that Wnt signaling pathway may be one of regulation pathways in periodontal tissue remodeling during OTM.

Key words: Wnt3a , DKK1 , Orthodontic tooth movement , Periodontal tissue remodeling

CLC Number:

R783.5

LU Juan, DUAN Ying-ying, WU Ming-ming, et al.. Expression of Wnt3a and DKK1 during Orthodontic Tooth Movement.[J]. Journal of Oral Science Research, 2015, 31(5): 448-450.

References

[1] Galli C, Passeri G, Macaluso GM. Osteocytes and WNT: the mechanical control of bone formation [J]. Journal of dental research, 2010, 89(4)∶331-343
[2] Dhamdhere GR, Fang M Y, Jiang J, et al. Drugging a stem cell compartment using Wnt3a protein as a therapeutic [J]. PloS one, 2014, 9(1)∶e83650[3] 王露霏,白丁,韩向龙.Wnt 信号通路拮抗Dkkl调节骨代谢的最新进展[J].中国组织工程研究,2013(2)∶337-341
[4] Lim WH, Liu B, Mah S, et al. Alveolar Bone Turnover and Periodontal Ligament Width are Controlled by Wnt [J]. Journal of periodontology, 2014(0)∶1-14
[5] Kim JY, Kim BI, Jue SS, et al. Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats [J]. The Angle orthodontist, 2011, 82(1)∶107-114
[6] Butler JS, Murray DW, Hurson CJ, et al. The role of Dkk1 in bone mass regulation: correlating serum Dkk1 expression with bone mineral density [J]. Journal of Orthopaedic Research, 2011, 29(3)∶414-418
[7] Napimoga MH, Nametala C, Silva FL, et al. Involvement of the Wnt/β‐catenin signaling antagonists, sclerostin and dickkopf‐related protein 1, in chronic periodontitis [J]. Journal of clinical periodontology, 2014, 41(6)∶550-557
[8] Fujita K, Janz S. Attenuation of WNT signaling by DKK-1 and-2 regulates BMP2-induced osteoblast differentiation and expression of OPG, RANKL and M-CSF [J]. Molecular cancer, 2007, 6(1)∶71
[9] 刘宁,冯云霞,陈显久.不同正畸力对大鼠牙周组织压力区核因子κB受体活化因子配基表达影响研究[J].中国实用口腔科杂志,2012,5(2)∶104-108
[10] Hamamura K, Chen A, Nishimura A, et al. Predicting and validating the pathway of Wnt3a-driven suppression of osteoclastogenesis [J]. Cellular signalling, 2014, 26(11)∶2358-2369
[11] Xu H Y, Ning DD, Liu RF, et al. Contributed Mini Review: Role of Wnt signaling in fracture healing [J]. Biochemistry and Molecular Biology Reports, 2014, 47(12)∶668-674
[12] 李昕,朱智敏.Wnt 信号通路与骨细胞生物力刺激信号转导间的关系[J].国际口腔医学杂志, 2012, 3∶015
[13] Aiko N, Masato T. Regulation of matrix metalloproteinase-13 and tissue inhibitor of matrix metalloproteinase-1 gene expression by WNT3A and bone morphogenetic protein-2 in osteoblastic differentiation [J]. Frontiers in Bioscience, 2006, 11∶1667-1678
[14] Canavarro C, Teles R P, Júnior J C. Matrix metalloproteinases-1,-2,-3,-7,-8,-12, and-13 in gingival crevicular fluid during orthodontic tooth movement: a longitudinal randomized split-mouth study [J]. The European Journal of Orthodontics, 2013, 35(5)∶652-658
[15] Niida A, Hiroko T, Kasai M, et al. DKK1, a negative regulator of Wnt signaling, is a target of the β-catenin/TCF pathway [J]. Oncogene, 2004, 23(52)∶8520-8526