Abstract: Objective: To investigate the effect of DEMPs on proliferation and differentiation of SHED in vitro and to provide theoretical basis for the application of DEMPs in tooth reparation and regeneration. Methods: The stem cells were collected from human exfoliated deciduous teeth by enzyme digestion and tissue ex-plant method. We assessed the multi-differentiation potentials of the cells by inducement. Healthy teeth wereextracted from cattles, and prepared dentine matrix of the teeth was extracted by 4mol/L guanidine hydrochloride and 0.5mol/L EDTA. The effect of DEMPs on the proliferation activity of SHED was evaluated by MTT method and the effect ofDEMPs on the activity of ALP was also examined. The expression of DMP-1 and DSPPinduced by DEMPs observed by RT-PCR. Results: The proliferation of SHED was induced by DEMPs after three and five days, and increased activity of ALP was induced by DEMPs after five and seven days. The mRNA expression of DSPP and DMP-1 in SHED induced by DEMPs. Conclusion: DEMPs can promote the proliferation and the odontogenic differentiation of SHED.