Journal of Oral Science Research

Journal of Oral Science Research ›› 2017, Vol. 33 ›› Issue (8): 807-810.DOI: 10.13701/j.cnki.kqyxyj.2017.08.002

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Cultivation, Identification and Multiple Differentiation of Rabbit Adipose-derived Stem Cells.

WANG Lin1, WU Guo-min1*, SUN Xiu-mei2, XIAO Yan-ju1, CHEN Kai1, PENG Pei-xuan1.   

  1. 1. Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
    2. Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China.
  • Received:2017-02-15 Online:2017-08-20 Published:2017-08-28

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Abstract: Objective: To isolate, cultivate, identify, and multipotential differentiate the rabbit adipose-derived stem cells. Methods: Adipose tissue was taken from New Zealand white rabbits groin and the cells were obtained. The cells were cultured and observed. Phenotypic markers and differentiation characteristics of cells were evaluated. Results: Cells obtained were kind of fibroblast-like cells which grew rapidly. The cells could proliferate stably up to 20 passages or more. The cell surface antigens CD34 and CD14 were negative, while CD44 were positive. In addition, they could differentiate into osteoblasts, adipocytes, and chondrocytes. Conclusion: ADSCs were isolated successfully. The cells proliferated in vitro steadily and had good multiple differentiation potential.

Key words: Adipose-derived stem cells , Multiple differentiation

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