Abstract: Objective: To observe the effects of different materials on remineralization of demineralized enamel caused by carbonated beverage. Methods: Extracted premolars for orthodontic purpose were randomly divided into demineralized group (group A), distilled water treatment group (group B), and penetrating resin treatment group (group C). All teeth were divided into mesial and distal enamel blocks, which were soaked in sprite to establish the demineralization model. Then, the enamel surfaces of group A were covered with acid-resistant nail polish, and the enamel surfaces of group B and group C were treated with distilled water and penetrating resin, respectively. All enamel blocks were placed in artificial saliva for remineralization at 37℃ room temperature water bath for 8 weeks. Six mesial enamel blocks were respectively randomly selected in the A&B&C group. The microstructures of the enamel surface were observed under scanning electron microscope. The fluorescence images of remaining mesial enamel blocks were taken under the laser scanning confocal microscope. The micro-Vickers hardness test was used to evaluate the hardness of distal enamel blocks. Results: Under scanning electron microscope, the enamel surfaces of group A were rough and unequal size pore structure formed by a large number of fractured enamel rod. Enamel surfaces of group B showed a small amount of mineral deposition, and some pores became shallow or disappeared. Enamel surfaces of group C were smooth with no obvious pores. Under the laser scanning confocal microscope, the fluorescence area and intensity of group A were strong, and the fluorescence area and intensity of group B and group C were decreased, and the degree of attenuation in group C was more obvious than that in group B. The microhardness value of group B and group C was higher than that of before remineralization, and the increased value of group C was higher than that of group B (P<0.05). Conclusion: Penetrating resin has a positive therapeutic effect on experimental and demineralized enamel caused by carbonated beverage.

Key words: Penetrating resin, Carbonated beverage , Demineralized enamel , Remineralization