Effect of YAP1 Small Interfering RNA on Biological Characteristics of Tongue Squamous Cell.

doi:10.13701/j.cnki.kqyxyj.2017.12.010

Abstract

Abstract: Objective: To investigate the effect of YAP1 small interfering RNA (siRNA) on the biological characteristics of tongue squamous cell. Methods: Human tongue cancer SCC-4 cells were cultured. According to the different transfectants, the cells were divided into siRNA-YAP1 group, siRNA-control sequence group, and blank control group. The expression of YAP1 gene in each group was detected by using real-time fluorescence quantitative PCR. The expression of YAP1 protein in each group was detected by Western blot. The cell proliferation was detected by MTT assay. The cell apoptosis in each group was detected by flow cytometry. The cell migration ability was detected by scanning experiment. The cell invasion ability in each group was detected by Transwell method. Results: Compared with the siRNA-control sequence group and the blank control group, the relative expression levels of YAP1 mRNA and protein in siRNA-YAP1 group decreased (P<0.05). Compared with the siRNA-control group and the blank control group, the absorbance A values at 24h, 48h, 72h, and 96h in the siRNA-YAP1 group were decreased (P<0.05). Compared with the siRNA-control sequence group and the blank control group, the apoptosis rate in the siRNA-YAP1 group was increased (P<0.05). Compared with the siRNA-control sequence group and the blank control group, the number of invasive cells in the siRNA-YAP1 group was decreased (P<0.05). Conclusion: Specific silencing of YAP1 gene in human tongue cancer SCC-4 cells could inhibitell proliferation, accelerate cell apoptosis, and reduce cell migration and invasion.

Key words: Tongue squamous cell carcinoma, YAP1, Small interfering RNA, Biological characteristics

CLC Number:

R739.8

WU Chang-jing, WANG Wei-Lu, ZHEN En-ming, SHAO Jun.. Effect of YAP1 Small Interfering RNA on Biological Characteristics of Tongue Squamous Cell.[J]. Journal of Oral Science Research, 2017, 33(12): 1278-1281.

References

[1] Thavaraj S, Cobb A, Kalavrezos N, et al. Carcinoma cuniculatum arising in the tongue [J]. Head Neck Pathol, 2012, 6(1)∶130-134
[2] Ling W, Mijiti A, Moming A. Survival pattern and prognostic factors of patients with squamous cell carcinoma of the tongue: a retrospective analysis of 210 cases [J]. J Oral Maxillofac Surg, 2013, 71(4)∶775-785
[3] Moroishi T, Hansen CG, Guan KL. The emerging roles of YAP and TAZ in cancer [J]. Nat Rev Cancer, 2015, 15(2)∶73-79
[4] Bae JS, Kim SM, Lee H. The Hippo signaling pathway provides novel anti-cancer drug targets [J]. Oncotarget, 2017, 8(9)∶16084-16098
[5] Mo JS. The role of extracellular biophysical cues in modulating the Hippo-YAP pathway [J]. BMB Rep, 2017, 50(2)∶71-78
[6] Guo C, Wang X, Liang L. LATS2-mediated YAP1 phosphorylation is involved in HCC tumorigenesis [J]. Int J Clin Exp Pathol, 2015, 8(2)∶1690-1697
[7] 王江,王旭东,赵凤仪,等.YAP1在人肺腺癌细胞厄洛替尼耐药中的作用[J].军事医学,2016,40(1)∶35-39
[8] Wierzbicki PM, Rybarczyk A. The Hippo pathway in colorectal cancer [J]. Folia Histochem Cytobiol, 2015, 53(2)∶105-119
[9] 张宁,王玉,王智勇.Yes相关蛋白1与肿瘤相关性的研究进展[J].医学综述,2015,21(1)∶47-50
[10] 王君莲,张绪,毛亮,等.CDH1基因在SD大鼠舌黏膜癌变过程中甲基化和突变的研究[J].口腔医学研究,2016,32(2)∶126-130
[11] 毛静月,刘蕊,李彩云,等.siRNA靶向沉默YAP1对卵巢癌细胞skov3增殖凋亡的影响[J].中国老年学杂志,2017,37(4)∶797-799
[12] Zhi X, Zhao D, Zhou Z, et al. YAP promotes breast cell proliferation and survival partially through stabilizing the KLF5 transcription factor [J]. Am J Pathol, 2012, 180(6)∶2452-2461
[13] 朱世凯,周玉,汪旭东,等.特异性siRNA沉默YAP1基因表达对胰腺癌细胞恶性生物学行为的影响[J].中华普通外科杂志,2014,29(9)∶719-722

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