Journal of Oral Science Research ›› 2018, Vol. 34 ›› Issue (9): 991-994.DOI: 10.13701/j.cnki.kqyxyj.2018.09.017

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Effects of Quercetin on Human Oral Keratinocyte Regeneration.

Miyashaer·Hujiahemaiti, LI Xue, LV Hui-xin, DING Xin-xin, ZHOU Yan-min*.   

  1. Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021,China.
  • Received:2018-03-16 Online:2018-09-28 Published:2018-09-25

Abstract: Objective: To assess the potential of quercetin on gingival regeneration. Methods: Cell viability and proliferation capacity were tested by CCK-8 kit. Cell migration was monitored using Transwell chambers. An inflammatory situation was mimicked using Pg. LPS. RT-qPCR was performed to measure mRNA levels of IL-β1, TNF-α, TGF-β1, and TGF-β3. Protein levels of TGF-β1 and TGF-β3 were evaluated with ELISA. Results: 20 μmol/L quercetin enhanced the proliferation and migration of HOKs. Furthermore, 20 μmol/L quercetin increased both mRNA and protein levels of TGF-β3 under inflammatory conditions while 50 μmol/L quercetin increased expression of TGF-β1. Expression of IL-β1 and TNF-α were downregulated by quercetin treatment. Conclusion: Compared with untreated controls, the re-epithelialization of 20 μmol/L quercetin-treated HOKs were highly rapid.