Effects of Calcium Hydroxide on Stem Cells Proliferation from Apical Papilla of Human.

doi:10.13701/j.cnki.kqyxyj.2018.09.016

Abstract

Abstract: Objective: To investigate the effect of calcium hydroxide (CH) on proliferation of stem cells from apical papilla (SCAPs) of young human. Methods: The human SCAPs were isolated and cultivated by enzyme digestion method combined with adherent explanted tissue culture. According to the concentration of CH extract, it is divided into six experimental groups. The cultivation was terminated after 1, 3, 5, 7, and 9 days. The proliferation was determined by MTT method. Results: Compared with the control group, the proliferation of SCAPs in the 0.1%, 0.5%, 1.0%, and 5.0% groups was not significantly different at day 5 (P>0.05), however, the proliferation in the 10.0% and 15.0% groups was inhibited (P<0.05). At day 7 and 9, the proliferation of all experimental groups was inhibited (P<0.05). Conclusition: CH inhibits the proliferation of human SCAPs, which is enhanced with time elapse. The inhibitory effect of CH on human SCAPs proliferation is more obvious at high concentration.

Key words: Stem cells from the apical papilla , Calcium hydroxide , Cell culture, Identification, Proliferation

YANG Wei-hong, XIE Ru, LIU Yao, LIU Xing-rong.. Effects of Calcium Hydroxide on Stem Cells Proliferation from Apical Papilla of Human.[J]. Journal of Oral Science Research, 2018, 34(9): 987-990.

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