Correlation between Methyltransferase DNMT1 and TCDD-induced Cleft Palate in Mice

doi:10.13701/j.cnki.kqyxyj.2019.02.007

Abstract

Abstract: Objective : To investigate the correlation between DNMT1 and TCDD-induced cleft palate in mice by detecting the expression of DNMT1 in TCDD-induced cleft palate mice. Methods: TCDD could cause fetus rats cleft palate by toxic effects on palatal medial edge epithelial cells. C57BL/6J pregnant mice were treated with TCDD (64 μg/kg) at day 10.5 (E10.5) to be the modeling. Oral tissues of E13.5, E14.5, E15.5, and E17.5 fetal rats were obtained, and HE staining was used to detect the occurrence of fetal cleft palate. Reverse transcription quantitative polymerase chain reaction was used to detect the changes of mRNA expression in DNMT1 of fetal rats. Western blot was used to detect the expression level of DNMT1 protein. Results: HE staining showed that the incidence of cleft palate in group TCDD was 56/57, and there was no cleft palate in control group. RT-qPCR results showed that DNMT1 mRNA in TCDD group was significantly higher than that in control group (P<0.01). Western blot results showed that compared with the control group, the expression level of DNMT1 protein in TCDD group was significantly higher (P<0.01). Conclusion: Methyltransferase DNMT1 plays an important role in the process of TCDD induced cleft palate formation in fetal rats.

Key words: Nonsyndromic cleft, lip with or without cleft palate, Methylation, Tetrachlorodibenzo-P-dioxin, DNA methyltransferase 1

LI Yong, ZHANG Wei, AN Li, SONG Hong-quan, ZHANG Xing-wei, GAO Yu-wei, ZANG Qi-guang, JIAO Xiao-hui.. Correlation between Methyltransferase DNMT1 and TCDD-induced Cleft Palate in Mice[J]. Journal of Oral Science Research, 2019, 35(2): 129-132.

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