Effects of Unilateral Chewing on Structure of Deep Masseter Muscle in Rats and Regulation Mechanism of IGF-1

doi:10.13701/j.cnki.kqyxyj.2019.11.020

Abstract

Abstract: Objective: To investigate the effect of unilateral chewing on the structure of deep masseter in rats and the role of IGF-1. Methods: Twenty-four male Wistar rats aged 4 weeks were randomly divided into experimental group and control group. A unilateral chewing model was established by unilateral occlusal plate. After 1, 2, 3, and 4 weeks of modeling, the deep masseter muscle was taken and the structural changes were observed by frozen section HE, ATPase, and NADH-TR staining. The expression of IGF-1 and CaN mRNA was detected by real-time RCR. The level of IGF-1 protein was detected by ELISA and the CaN protein was detected by Western blot. Results: After 2, 3, and 4 weeks of wearing occlusal plate, the proportion of MHCⅡa fibers on the non-biased chewing side increased more than that of the chewing side and was higher than that of the control group (P<0.05). The protein and mRNA content of IGF-1 and CaN on the non-biased chewing side were higher than that of the non-biased chewing side and control group at week 3 and 4 (P<0.05). Conclusion: Unilateral chewing leads to the transition of muscle fiber type from MHCⅡb to MHCⅡa, which may relate to the increased secretion of IGF-1 and CaN.

Key words: unilateral chewing, masseter, insulin-like growth factor-1, calcineurin

LIU Siqi, LI Yinghui, LIU Ziyang, WANG Wen, MA Wensheng. Effects of Unilateral Chewing on Structure of Deep Masseter Muscle in Rats and Regulation Mechanism of IGF-1[J]. Journal of Oral Science Research, 2019, 35(11): 1094-1099.

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