Effects of Combined Application of BMP-2 and TGF-1α on Bone Formation in Maxillary Sinus Lifting in Dogs

doi:10.13701/j.cnki.kqyxyj.2020.09.012

Abstract

Abstract: Objective: To test the effects of BMP2 and TGF-1α on bone differentiation of canine bone marrow stem cells in vitro, and verify it in vivo. Methods: BMP2 and TGF-1α were overexpressed by constructing lentivirus, and then combined with BIOSS bone meal and implanted into the maxillary sinus of dogs. New bone formation and biological stability (by ISQ) were detected. Results: Bone formation in the combined application of BMP2 and TGF-1α group was significantly higher than those of other groups, and the same as biological stability. Conclusion: The application of BMP2 and TGF-1α can significantly promote bone formation, which has clinical application prospects.

Key words: BMP2, TGF-1α, composite material, maxillary sinus

MA Dong, LIU Xin, LIU Yajun, DING Ruyuan, YANG Gang, CHEN Yongxiang, WANG Yuanyin. Effects of Combined Application of BMP-2 and TGF-1α on Bone Formation in Maxillary Sinus Lifting in Dogs[J]. Journal of Oral Science Research, 2020, 36(9): 850-854.

References

[1] Levi B, Péault B, James AW. Bone tissue engineering and regeneration [J]. Biomed Res Int, 2014, 2014:137529.
[2] Rau JV, Antoniac I, Cama G, et al. Bioactive materials for bone tissue engineering [J]. Biomed Res Int, 2016, 2016:3741428.
[3] 张悦,胡杨,沈玉凤,等.bFGF/BMP-2转染大鼠骨髓干细胞体内成牙的研究[J].口腔医学研究,2016,32(3):239-243.
[4] 林玮民,陈苗,胡琛,等.骨形成蛋白-2与碱性成纤维细胞生长因子联合应用于骨组织工程研究进展[J].口腔疾病防治,2018,26(5):325-329.
[5] 周俊,王兆晶,陈文瑨,等.血管内皮生长因子和骨形成蛋白2诱导犬恒牙原位牙髓再生[J].中华口腔医学研究杂志,2016,10(1):5-10.
[6] 郭铁峰,周明旺,李盛华,等.转化生长因子β1对软骨组织代谢影响的研究进展[J].中国组织工程研究,2013,17(15):2827-2834.
[7] 王劲,张勇,罗成基,等.骨髓间充质干细胞成骨分化中转化生长因子受体的表达[J].中国组织工程研究与临床康复,2009,13(6):1049-1052.
[8] Pei GX, Qin YX, Hutmacher DW, et al. Bone tissue engineering: cell motility, vascularization, micro-nano scaffolding, and remodeling [J]. Biomed Res Int, 2014, 2014:286978.
[9] Xiao Y, Bone tissue engineering for dentistry and orthopaedics [J]. Biomed Res Int, 2014, 2014:241067.
[10] Miura KI, Sumita Y, Kajii F. First clinical application of octacalcium phosphate collagen composite on bone regeneration in maxillary sinus floor augmentation: A prospective, single-arm, open-label clinical trial [J]. Biomed Mater Res B Appl Biomater, 2020, 108(1):243-252.
[11] Helder MN, van Esterik FAS, Kwehandjaja MD. Evaluation of a new biphasic calcium phosphate for maxillary sinus floor elevation: Micro-CT and histomorphometrical analyses [J]. Clin Oral Implants Res, 2018, 29(5):488-498.
[12] Xiang Y, Yao X, Wang X. Houshiheisan promotes angiogenesis via HIF-1α/VEGF and SDF-1/CXCR4 pathways: in vivo and in vitro [J]. Biosci Rep, 2019, 39(10):BSR20191006.
[13] Xue L, Huang J, Zhang T. PTEN inhibition enhances angiogenesis in an in vitro model of ischemic injury by promoting Akt phosphorylation and subsequent hypoxia inducible factor-1α upregulation [J]. Metab Brain Dis, 2018, 33(5):1679-1688.

[1]	GENG Shuoshuo, LIU Xin, GUO Shouli, LI Ying, GUAN Xue, WANG Xiumei, XIE Xiaohua. Deletion of Bmp2 and Bmp4 from Epithelium Affected Tooth Root Development in Mice [J]. Journal of Oral Science Research, 2020, 36(8): 726-730.
[2]	XU Laijun, LI Jiyao. Research Progress on Gellan Gum Composite in the Field of Bone Defect [J]. Journal of Oral Science Research, 2020, 36(6): 512-515.
[3]	WANG Tengfei, XU Yan, XU Hanying, HAN Xu, SHEN Jilong, CHENG Ting. Preparation of Yolk Antibody Sustained-release Nano-hydroxyapatite and Its Antibacterial Properties [J]. Journal of Oral Science Research, 2020, 36(6): 591-594.
[4]	ZHANG Lijuan, JIANG Ming, LI Shanshan, LI Fan, ZHANG Ying, TAN Kaixuan, LU Jie, YANG Fang. Acute Apical Periodontitis of Primary Molar with Odontogenic Maxillary Sinusitis and Infection of Infraorbital Space: A Case Report [J]. Journal of Oral Science Research, 2020, 36(1): 87-88.
[5]	YANG Shi-hui, ZHAO Jing-hui. Complications and Management of Maxillary Sinus Floor Elevation in Elderly Patients [J]. Journal of Oral Science Research, 2019, 35(3): 227-229.
[6]	YU Wan-qi, YANG Shi-hui, ZHOU Zhe, ZHOU Yan-min, ZHAO Jing-hui. Clinical Study on Minimally Invasive Maxillary Sinus Elevation with Simultaneous Implantation in Elderly Patients [J]. Journal of Oral Science Research, 2019, 35(3): 258-261.
[7]	WANG Han-chi,ZHAO Jing-hui,LIU Chun-ying,WANG Nan,WANG Yao,ZHOU Yan-min. A Review on Recent Progress of Platelet Rich Fibrin in Maxillary Sinus Elevation [J]. Journal of Oral Science Research, 2018, 34(6): 591-593.
[8]	HE Xiao-ji, LIU Dang-li, SUN Gui-feng, CHENG Tao. Clinical Study of Maxillary Sinus Floor Elevation through Controlling the Tension of Maxillary Sinus Mucosa [J]. Journal of Oral Science Research, 2018, 34(6): 666-670.
[9]	CUI Ting-ting, KOU Ni, ZHONG Wei-jian, MA Guo-wu. Application of Autologous Dentine Granules and Platelet Rich Fibrin in Maxillary Sinus Lateral Lift Surgery. [J]. Journal of Oral Science Research, 2018, 34(5): 532-534.
[10]	WANG Na, QU Zhe, ZHOU Hong-zhi, CHEN Xiao-dong. Clinical Assessment of CGF in the Maxillary Sinus Floor Lift [J]. Journal of Oral Science Research, 2018, 34(2): 186-189.
[11]	ZHONG Zhi-hua, TANG Yi, SHI Bin.. Application of Bicon Implant and Maxillary Sinus Inner Lift Technology for Upper Posterior Missed Teeth. [J]. Journal of Oral Science Research, 2017, 33(7): 787-789.
[12]	SONG Juan, ZHANG Zhi-qing, KANG Hong-yu, CAO Yan-ying, MA Chen-lin, CHEN Ning.. Analysis of Maxillary Sinus Septa in Population of Qinghai by Cone-beam CT. [J]. Journal of Oral Science Research, 2017, 33(3): 282-285.
[13]	HU Pin, LI Yan-feng, REN Bo, FAN Jia-dong, ZHANG Ying, ZHANG Yue, HAN Yi-shi,WANG Ning.. Effects of Four Kinds of Stripping Devices on Stripping Lengths of Flat Maxillary Sinus Floor Mucosa of Goat in Vitro. [J]. Journal of Oral Science Research, 2017, 33(2): 162-165.
[14]	Ying,QU Zhe,MA Lan,HAN Wen-li. Anatomical Presentation of Sinus Membrane and Ostium in Cone Beam Computed Tomographic Scan [J]. Journal of Oral Science Research, 2016, 32(8): 853-856.
[15]	GAO Wei, LI Liang-yu, ZHANG Feng.. Two Minimally Invasive Maxillary Sinus Lifting and Simultaneously Placement of Implants from the Top of Alveoli with 3~5 Years Clinic follow-up Study. [J]. Journal of Oral Science Research, 2016, 32(6): 643-647.