Decursin Reduces Periapical Inflammation by Inhibiting OPN Expression

doi:10.13701/j.cnki.kqyxyj.2021.03.007

Abstract

Abstract: Objective: To study the effects and mechanism of decursin in periapical periodontitis. Methods: The rat periapical periodontitis model was established and divided into normal control group, model observation group, and apical periodontitis group. The rats in the apical periodontitis group were equally divided into the decursin group (given decursin, 20mg/kg per day) and normal saline group (given the same dose of saline) in the first week, respectively. The rats were sacrificed in the third week. The area of the periapical lesions was analyzed by radiographs and HE staining. The expression of OPN in the alveolar bone was detected using immunohistochemical staining. Mouse mononuclear macrophages RAW264.7 cells were cultured and treated with LPS and decursin. The proliferation and the level of IL-6 were detected by CCK-8 assay and ELISA, respectively. The expression of OPN was examined by western blot. Results: The periapical lesion areas of the control group were remarkably higher than those of the decursin groups. Decursin decreased the protein levels of OPN. The expression of OPN, the proliferation ability, and the level of IL-6 of RAW264.7 cells were increased after treated with LPS. Decursin inhibited these effects. Conclusion: Decursin suppressed the expression of OPN and inhibited the progress of the periapical periodontitis.

Key words: decursin, periapical periodontitis, OPN

ZHENG Lijuan, LI Haoran, LIU Jianwei, HAN Bing, CHEN Xiaodong. Decursin Reduces Periapical Inflammation by Inhibiting OPN Expression[J]. Journal of Oral Science Research, 2021, 37(3): 213-217.

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