Expression of RIP1, RIP3 and MLKL in Inflammatory Periodontal Tissue under Orthodontic Force

doi:10.13701/j.cnki.kqyxyj.2021.10.009

Abstract

Abstract: Objective: To study the expression of RIP1, RIP3, and MLKL in the pressure-side periodontal tissue during orthodontic tooth movement in rats and to explore its role in pressure-side periodontal tissue remodeling. Methods: Thirty healthy male SD rats were selected as the research object in this experiment and randomly divided into 6 groups: 0 d, 1 d, 3 d, 5 d, 7 d and 14 d groups, with 5 rats in each group. 0 d group was the blank control group. The two central incisors of the upper jaw were anchors, and the left first molar was pulled forward with a force value of 50 g by a nickel-titanium tension spring. After the rats were sacrificed at each time point, the first molar and the surrounding periodontal tissues were collected, and the expression levels of RIP1, RIP3, and MLKL on the pressure side were detected by immunohistochemistry. Results: Compared with the blank control group, the expression of RIP1, RIP3, and MLKL in the experimental group increased at first and then decreased, and the expression at each time point was higher than that in the blank control group. The expression increased significantly on the 7th day. Conclusion: There was necroptosis in the pressure side during the orthodontic tooth movement, and RIP1, RIP3 and MLKL were both involved in the periodontal tissue remodeling on the pressure side during orthodontic tooth movement.

Key words: orthodontics, tooth movement, periodontium remodeling, pressure side

ZHANG Minjie, ZHANG Miaomiao, JI Kaixin, CHEN Siyan. Expression of RIP1, RIP3 and MLKL in Inflammatory Periodontal Tissue under Orthodontic Force[J]. Journal of Oral Science Research, 2021, 37(10): 906-909.

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