Bone Regeneration Effects of Tooth Ash in Conjunction with Platelet-rich Fibrin in an Animal Mode

Abstract

Abstract: Objective: To assess the initial effects of tooth ash in conjunction with platelet-rich fibrin (PRF) on reconstruction of bone defect. Methods: Ten white rabbits were selected to create an animal model with four defects on the skull. Defects in group A was used as vacant control, tooth ash particles were used solely in group B, tooth ash mixed with PRF were used in group C, and the defects in group D were filled with tooth ash combined with bone marrow. The animals were sacrificed 6 weeks later, bone samples were harvested for histological observation and statistical analysis. Results: The percentage of new bone formed in each group was 7.46%±2.21%, 17.61%±2.29%, 21.00%±4.73%, and 23.42%±3.22%, respectively. Group C and D showed a statistically greater amount of new bone formation compared with group A and B (P<0.05). No significant differences were found between group C and D. Conclusion: Teeth ash particle is a type of bone graft material with favorable biocompatibility and osteoconductivity. After in conjunction with PRF, it can facilitate the bone healing process in early stage.

CLC Number:

R782.13

GUO Jin-yuan, ZHONG Wei-jian, CHAI Song-ling, LIANG Xin, MA Guo-wu.. Bone Regeneration Effects of Tooth Ash in Conjunction with Platelet-rich Fibrin in an Animal Mode[J]. Journal of Oral Science Research, 2015, 31(11): 1069-1072.

References

[1] Reena AB, Tamara DR. Bone Graft Substitutes [J]. Hand Clinics, 2012, 28(4)∶457-468
[2] Kim WB, Kim SG, Lim SC, et al. Effect of Tisseel on bone healing with particulate dentin and plaster of Paris mixture [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2010, 109(2)∶34-40
[3] Hara S, Mitsugi M, Kanno T, et al. Bone Transport and Bone Graft Using Auto-Tooth Bone for Alveolar Cleft Repair [J]. J Craniofac Surg, 2013, 24(1)∶65-8
[4] 张国锋,庞永志.骨移植材料在局部牙槽骨缺损中的应用进展[J].中国现代医药杂志,2013,15(3)∶117-120
[5] 刘昌奎,谭新颖,罗金超,等.同种异体骨支架复合自体骨髓间充质干细胞修复下颌骨节段缺损动物模型的建立[J].口腔医学研究,2015,(1)∶27-30
[6] Kim BK, Kim SG, Kim SY, et al. A comparison of bone generation capability in rabbits using tooth ash and plaster of Paris with platelet-rich plasma or fibrin sealant [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2010, 110(3)∶8-14
[7] Kim YK, Kim SG, Oh JS, et al. Analysis of the Inorganic Component of Autogenous Tooth Bone Graft Material [J]. J Nanosci Nanotechnol, 2011, 11(8)∶7442-5
[8] Miyata Y, Ozawa S, Kojima N, et al. An experimental study of bone grafting using rat milled tooth [J]. Int J Oral Maxillofac Implants, 2011, 26(6)∶1210-6
[9] 李琦,周延民,翟静捷,等.富血小板纤维蛋白修复兔颅骨骨缺损的实验研究[J].口腔医学研究,2011,27(7)∶587-589
[10] Sclafani AP, Saman M. Platelet-rich fibrin matrix for facial plastic surgery[J]. Facial Plast Surg Clin North Am, 2012, 20(2)∶117-186
[11] Smita Singh, Arunendra Singh, Sourav Singh, et al. Application of PRF in surgical management of periapical lesions [J]. Natl J Maxillofac Surg, 2013, 4(1)∶94-99
[12] Jeong KI, Kim SG, Kim YK, et al. Clinical study of graft materials using autogenous teeth in maxillary sinus augmentation [J]. Implant Dent, 2011, 20(6)∶471-475
[13] Dohan DM, Choukroun J, Diss A,et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part II: Platelet-related biologic features [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006, 101(3)∶45-50
[14] Dohan Ehrenfest, David M, Doglioli, et al. Choukroun's platelet-rich fibrin (PRF) stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way [J]. Arch Oral Biol, 2010, 55(3)∶185-194
[15] Lee JW, Kim SG, Kim JY, et al. Restoration of a peri-implant defect by platelet-rich fibrin [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2012, 113(4)∶469-463
[16] Marrelli M; Tatullo M. Influence of PRF in the healing of bone and gingival tissues. Clinical and histological evaluations [J]. Eur Rev Med Pharmacol Sci, 2013, 17(14)∶1958-1962
[17] Friedenstein AJ, Chailakhyan RK,Gerasimov UV. Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers [J]. Cell Tissue Kinet,1987, 20(3)∶263-272
[18] Kassem MS. Percutaneous autogenous bone marrow injection for delayed union or non union of fractures after internal fixation [J]. Acta Orthop Belg, 2013, 79(6)∶711-717
[19] Rickert D, Vissink A, Slot W J, et al. Maxillary sinus floor elevation surgery with BioOss mixed with a bone marrow concentrate or autogenous bone: test of principle on implant survival and clinical performance [J]. Int J Oral Maxillofac Surg , 2014, 43(2)∶243-247
[20] 顾九君,陶若奇,梁伟东,等.自体骨髓经皮注射移植治疗胫骨骨不连的临床效果观察[J].甘肃科技, 2014, 30(13)∶153-154
[21] Connolly JF, Shindell R. Percutaneous marrow injection for an ununited tibia[J]. Nebr Med J, 1986, 71(4)∶105-107
[22] 梁雨田,张伯勋,卢世璧,等.经皮自体骨髓移植治疗骨折不愈合[J].中华骨科杂志,1999,(12)∶709-711