Abstract: Objective: To explore the root canal filling effect of the innovative negative pressure obturation technique and assess its clinical practicability. Methods: Freshly extracted teeth were collected and randomly allocated into 4 groups after root canal preparation: (A) the innovative negative pressure obturation group, (B) the single-cone filling group, (C) the cold lateral compaction group, and (D) the warm vertical compaction group. X-rays were taken after the operation, and scored the length, density, and taper of the root fillings. Cone beam computed tomography (CBCT) scans were conducted to measure and score the distance from the end of the root filling material to the radiographic apical foramen; teeth and root canals were screened to calculate the proportion of root filling bubbles and measure the maximum bubble diameter. Results: The X-ray outcomes indicated no statistically significant differences in the clinical scores of length, density, and taper among different filling techniques (P>0.05). The CBCT results demonstrated no significant difference in the distance from the end of the root filling material to the radiographic apical foramen among different filling methods (P>0.05), while different filling procedures had a significant impact on the proportion of bubbles in teeth and root canals (P<0.001). Conclusion: Based on imaging examinations, the innovative negative pressure obturation technique can achieve the same root canal filling effect as other root canal filling techniques and can serve as a non-inferior alternative treatment option for conventional root canal filling techniques.

Key words: endodontics, root canal treatment, root canal filling, innovative negative pressure obturation technique, CBCT