Abstract: Objective: To evaluate the feasibility of in vitro labeling human stem cells from deciduous pulp by CM-DiI. Methods: Enzyme tissue block method was used to culture human stem cells from pulp of deciduous teeth, and the biological characteristics of cells were identified. The third passage cells were labeled by 2μg/mL, 3μg/mL and 4μg/mL, respectively. The release of lactic dehydrogenase (LDH), proliferation and labeling rate were detected in vitro. Results: Human stem cells from deciduous pulp were harvested by enzyme tissue block method and immunohistochemistry staining confirmed the cells were derived from mesenchymal tissue, rather than from the epithelial tissue. Abilities of adipogenic and osteogenic/odontoblasts differentiation were verified after being induced. These three concentrations showed no significant adverse effect on cell proliferation and the release of LDH. Fluorescence intensity gradually decreased in vitro. Conclusion: The application of labeling human stem cells from pulp of deciduous teeth by CM-DiI is convenient and effective. 3μg/mL is the optimal concentration for labeling in the present study.

Key words: Human deciduous teeth pulp stem cells , CM-DiI , Cell labeling