Alizarin red and Alcian Blue Double Staining for the Whole Observation of Development of Mouse Mandible.

Abstract

Abstract: Objective: To explore the feasibility of using the alizarin red/ alcian blue double staining to observe the whole development of mouse mandible. Methods: Alcian blue and alizarin red solution were used to double stain the E15.5, E16.5, E18.5 and P90 mouse mandibular skeleton. We observed the whole development of mandible, especially focused on the Meckel's cartilage and condylar cartilage. Results: The bone and cartilage could be stained as red or blue respectively. The morphology of the developmental mandible could be clearly observed under the stereo microscope. The Meckel's cartilage was not tightly connected to the surrounding tissues. The condylar cartilage was independent of the Meckel's cartilage, and was tightly connected to the mandibular periosteum. Conclusion: Using the alizarin red/alcian blue double staining could successfully observe the whole development process of mouse mandible. The Meckel's cartilage may not directly take part in the bone formation of mandible, and the condylar cartilage may originate from the mesenchymal cell in tail end of mandible.

Key words: Alcian blue, Alizarin red , Double staining, Mandibuloar development, Whole observation

CLC Number:

R782.2

ZHAO Yi-song, ZHONG Hui-min, HE Zhi-wei, et al. Alizarin red and Alcian Blue Double Staining for the Whole Observation of Development of Mouse Mandible.[J]. Journal of Oral Science Research, 2015, 31(1): 15-17.

References

[1] Chai Y, Jiang X, Ito Y, et al. Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis [J]. Development, 2000,127(8)∶1671-1679
[2] Bhaskar SN, Weinmann JP, Schour I. Role of Meckel's cartilage in the development and growth of the rat mandible [J]. J Dent Res, 1953, 32(3)∶398-410
[3] Wyganowska-Swiatkowska M, Przystanska A. The Meckel's cartilage in human embryonic and early fetal periods [J]. Anat Sci Int, 2011, 86(2)∶98-107
[4] 华先明,程祥荣.下颌髁突软骨的结构和在拉伸/剪切负荷作用下的生物机械性能[J].口腔医学研究,2014,30(2)∶177-179
[5] Chai Y, Maxson RE, Jr. Recent advances in craniofacial morphogenesis [J]. Dev Dyn, 2006, 235(9)∶2353-2375
[6] Nakao K, Okubo Y, Yasoda A, et al. The effects of C-type natriuretic peptide on craniofacial skeletogenesis [J]. J Dent Res, 2013, 92(1)∶58-64
[7] Kamiya Y, Chen J, Xu M, et al. Increased mandibular condylar growth in mice with estrogen receptor beta deficiency [J]. J Bone Miner Res, 2013, 28(5)∶1127-1134
[8] Ramaesh T, Bard JB. The growth and morphogenesis of the early mouse mandible: a quantitative analysis [J]. J Anat, 2003, 203(2)∶213-222[9] Depew MJ, Simpson CA, Morasso M, et al. Reassessing the Dlx code: the genetic regulation of branchial arch skeletal pattern and development [J]. J Anat, 2005, 207(5)∶501-561
[10] Knight RD, Schilling TF. Cranial neural crest and development of the head skeleton [J]. Adv Exp Med Biol, 2006, 589∶120-133
[11] Tomo S, Ogita M, Tomo I. Development of mandibular cartilages in the rat [J]. Anat Rec, 1997,249(2)∶233-239
[12] Ishizeki K, Saito H, Shinagawa T, et al. Histochemical and immunohistochemical analysis of the mechanism of calcification of Meckel's cartilage during mandible development in rodents [J]. J Anat, 1999, 194 ( Pt 2)∶265-277
[13] Yang RT, Zhang C, Liu Y, et al. Autophagy prior to chondrocyte cell death during the degeneration of Meckel's cartilage [J]. Anat Rec (Hoboken), 2012, 295(5)∶734-741
[14] Vinkka-Puhakka H, Thesleff I. Initiation of secondary cartilage in the mandible of the Syrian hamster in the absence of muscle function [J]. Arch Oral Biol, 1993,38(1)∶49-54
[15] Shibata S, Fukada K, Suzuki S, et al. In situ hybridization and immunohistochemistry of bone sialoprotein and secreted phosphoprotein 1 (osteopontin) in the developing mouse mandibular condylar cartilage compared with limb bud cartilage [J]. J Anat, 2002, 200(Pt 3)∶309-320
[16] 张燕丽,张翠珍,彭刚,等.斑马鱼颅骨骨组织发育特征的研究[J].口腔医学研究,2014,30(2)∶97-99
[17] Alexander C, Piloto S, Le Pabic P, et al. Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton [J]. PLoS Genet, 2014,10(7)∶e1004479