Abstract: Objective: To investigate the miRNA expression in nonsyndromic cleft lip patients with or without cleft palate and its correlation with Wnt family in nonsyndromic cleft lip patients with or without cleft palate. Methods: Gene chips of MiRCURY^TM probe were used to detect and compare the microRNA expression of the lip and palate tissues in 4 NSCL/P patients as well as umbilical cord tissues in 4 normal newborns. Results: In total, 254 differentially expressed miRNAs were detected, among which 181 up-regulate and 73 down-regulate (P<0.05). According to the fold change and P value, the top ten different miRNAs were selected for target prediction. The results showed that hsa-miR-1260b, hsa-miR-205-5p, hsa-miR-24-3p, hsa-miR-27b-3p and hsa-miR-720 are combined with Wnt5a, Wnt9b and Wnt10a. Conclusion: Differentially expressed miRNA may control the development of lip and palate by targeting Wnt family in embryonic period.