Expression and Significance of Integrin-linked Kinase and Matrix Metalloproteinase-9 in Salivary Adenoid Cystic Carcinoma.

doi:10.13701/j.cnki.kqyxyj.2016.07.025

Abstract

Abstract: Objective: To investigate the expression and significance of integrin-linked kinase (ILK) and matrix metalloproteinase-9 (MMP-9) in salivary adenoid cystic carcinoma (SACC). Methods: 59 cases of SACC specimens and 42 cases of normal salivary gland tissues were collected. The expression of ILK and MMP-9 in SACC and normal tissues were detected by using QRT-PCR technology. Results: The mRNA expression levels of ILK and MMP-9 were significantly higher in SACC tissues than those in control group (P<0.05), and they were positively correlated in SACC tissues (r=0.586, P=0.000). The expression of ILK and MMP-9 in SACC tissues were correlated with disease subtype, lymph node metastasis and TNM stage (P<0.05). The mRNA expression levels of ILK and MMP-9 in entity and tubular type were obviously higher than sieve-like type (P<0.05). Conclusion: Up-regulation of ILK and MMP-9 may synergistically promote the invasion and metastasis of tumor cells in SACC.

Key words: Salivary adenoid cystic carcinoma, Integrin-linked kinase , Matrix metalloproteinase-9, Expression

CLC Number:

R739.8

JIN Yong-yong. Expression and Significance of Integrin-linked Kinase and Matrix Metalloproteinase-9 in Salivary Adenoid Cystic Carcinoma.[J]. Journal of Oral Science Research, 2016, 32(7): 764-767.

References

[1] Cai Q, Zhang R, Wu G, et al. Adenoid cystic carcinoma of submandibular salivary gland with late metastases to lung and choroid: a case report and literature review [J]. J Oral Maxillofac Surg, 2014, 72(9)∶1744-1755
[2] Ghatak S, Morgner J, Wickstrm SA. ILK: a pseudokinase with a unique function in the integrin-actin linkage [J]. Biochem Soc Trans, 2013, 41(4)∶995-1001
[3] Xiong D, Liou Y, Shu J, et al. Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway [J]. Exp Mol Pathol, 2014, 96(3)∶411-421
[4] Wang J, Shi Q, Yuan TX, et al. Matrix metalloproteinase 9 (MMP-9) in osteosarcoma: review and meta-analysis [J]. Clin Chim Acta, 2014, 433∶225-231
[5] Boukheris H, Curtis RE, Land CE, et al. Incidence of carcinoma of the major salivary glands according to the WHO classification, 1992 to 2006: a population-based study in the United States [J]. Cancer Epidemiol Biomarkers Prev, 2009, 18(11)∶2899-2906
[6] Thompson LD. Salivary gland adenoid cystic carcinoma [J]. Ear Nose Throat J, 2015, 94(7)∶262-264
[7] Qin J, Wu C. ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling [J]. Curr Opin Cell Biol, 2012, 24(5)∶607-613
[8] Piao Z, Hong CS, Jung MR, et al. Thymosin β4 induces invasion and migration of human colorectal cancer cells through the ILK/AKT/β-catenin signaling pathway [J]. Biochem Biophys Res Commun, 2014, 452(3)∶858-864
[9] Yan Z, Yin H, Wang R, et al. Overexpression of integrin-linked kinase (ILK) promotes migration and invasion of colorectal cancer cells by inducing epithelial-mesenchymal transition via NF-κB signaling [J]. Acta Histochem, 2014, 116(3)∶527-533
[10] Zheng K, Wang G, Li C, et al. Knockdown of ILK inhibits glioma development via upregulation of E-cadherin and downregulation of cyclin D1 [J]. Oncol Rep, 2015, 34(1)∶272-278
[11] Xin L, Hou Q, Xiong QI, et al. Association between matrix metalloproteinase-2 and matrix metalloproteinase-9 polymorphisms and endometriosis: A systematic review and meta-analysis [J]. Biomed Rep, 2015, 3(4)∶559-565
[12] Nascimento Dda C, Durigan Rde C, Tibana RA, et al. The response of matrix metalloproteinase-9 and -2 to exercise [J]. Sports Med, 2015, 45(2)∶269-278
[13] Wang L, Wang Y, Bian H, et al. Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability [J]. Oncol Rep, 2013, 30(1)∶207-212