Effect of P120ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma

doi:10.13701/j.cnki.kqyxyj.2016.11.007

Abstract

Abstract: Objective: To investigate the effect of P120^ctn silence and overexpression on cell migration and invasion of oral squamous cell cancer (OSCC). Methods: Plasmid pGFP-V-RS-P120^ctn shRNA was used to transfect HN4 cells, and plasmid pCMV6-AC-GFP-P120^ctn was used to transfect HN12 cells. Real-time PCR and Western Blot were performed to detect the mRNA and protein expression levels of P120^ctn and E-cad in OSCC cells before and after transfection. Trans-well assays were used to detect the invasion and migration abilities of OSCC cells before and after transfection. Results: After plasmid pGFP-V-RS-P120ctn shRNA transfection in HN4 cells, the expression levels of P120^ctn and E-cad were significantly decreased, while cell migration and invasion abilities were significantly increased (P<0.05). After plasmid pCMV6-AC-GFP-P120^ctn transfection in HN12 cells, the expression levels of P120^ctn and E-cad were significantly increased, while cell migration and invasion abilities were significantly decreased (P<0.05). Conclusion: P120^ctn may be involved in the invasion and metastasis by regulating the expression of E-cad and cell adhesion in OSCC.

Key words: P120-catenin, E-cadherin, Oral squamous cell cancer

CLC Number:

R739.8

CHEN Zhong, JI Qing, ZHENG Xiao-dan. Effect of P120^ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2016, 32(11): 1141-1145.

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Effect of P120^ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma

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