Effects of 1,25-dihydroxyvitamin D3 on Neutrophil Apoptosis and Inflammatory Mediators in Type 2 Diabetic Patients with Chronic Periodontitis.

doi:10.13701/j.cnki.kqyxyj.2017.03.009

Abstract

Abstract: Objective: To investigate the effect of 1,25 (OH)₂D₃ on neutrophils apoptosis and inflammatory mediators expression in Type 2 diabetic patients with chronic periodontitis. Methods: Neutrophils were isolated by density gradient centrifugation from Type 2 diabetic patients with chronic periodontitis peripheral blood and cultured in media with or without 1,25 (OH)₂D₃ for 24h. The apoptosis rate of neutrophils was detected by flow cytometry (FCM). Expressions of tumor necrosis factor-α, interleukin-1β, interleukin-6, and neutrophil elastase in supernatants were tested by enzyme-linked immunosorbent assay (ELISA). Results: Flow cytometry analysis showed that 1,25(OH)₂D₃ intervention group neutrophil apoptosis rate was increased significantly (P<0.05). Multiple comparison revealed that there were significant differences among groups with different concentrations (10^-8 mol/L intervention group>10^-6 mol/L intervention group>10^-10mol/L intervention group). ELISA results showed thattumor necrosis factor-α, interleukin -1β, and interleukin-6 content were decreased in 1,25(OH)₂D₃ intervention group (P<0.05). Conclusion: 1,25(OH)₂D₃ may promote the neutrophil apoptosis and decrease the expression of inflammatory mediators, and play an immune regulatory role in the occurrence and development in Type 2 diabetic patients with chronic periodontitis.

Key words: 1,25-dihydroxyvitamin , D3 Type 2 diabetes, Periodontitis, Neutrophils, Apoptosis

CLC Number:

R781.4

YAN Yan-mei, TANG Ya-ping, HE Tao, MA Can-can, FANG Hui, ZHONG Wen-yi, LIU Qi.. Effects of 1,25-dihydroxyvitamin D3 on Neutrophil Apoptosis and Inflammatory Mediators in Type 2 Diabetic Patients with Chronic Periodontitis.[J]. Journal of Oral Science Research, 2017, 33(3): 269-272.

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