Abstract: Objective: To explore the protein component changes in human placenta-derived mesenchymal stem cells (hPDMSCs) transfected with LIM mineralization protein-1 (LMP-1) gene using proteomics technologies. Methods: The hPDMSCsLMP-1 and hPDMSCsvector were collected to extract total proteins. The two-dimensional gel electrophoresis (2-DE) of total proteins in cells of two groups was created. The 2-DE map was analyzed and compared using PDQuest software to find out differentially expressed protein spots. These differentially expressed candidate proteins were identified by matrix-assisted laser desorption-ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS). Six protein spots (PCNA, vimentin, annexin A1, annexin A2, eEF2 and peroxiredoxin-1) which were closely related to osteogenesis and had a higher MALDI-TOF-MS-MS



基金项目 吉林省医药产业推进计划(编号:20140311088YY,201603028YY)
长春市重大科技攻关计划(编号:2014054)
吉林省科技攻关计划(编号:20150204004YY)
长春市“双十工程”计划(统带:16SS12)
作者简介 宋立杰(1990~ ),女,河北沧州人,硕士研究生,主要研究方向:口腔颌面部组织工程骨研究。
*通讯作者 刘志辉,E-mail:liuzhihui1975@sina.com
王博蔚,E-mail:wanbowei2009@hotmail.com

score were selected and detected by Western blotting. Results: The 2-DE maps of total proteins in cells were successfully established. Twenty-two differentially expressed protein spots with a difference in expression between hPDMSCsLMP-1 and hPDMSCsvector were identified by PDQuest 2-D Analysis Software. Combination of MALDI-TOF-MS and database search identified a total of 15 differentially expressed protein spots. Of all the identified protein spots, 9 proteins were up-regulated and 6 proteins were down-regulated in hPDMSCsLMP-1 as compared with the hPDMSCsvector. Western blot results were fully consistent with proteomics. Conclusion: Fifteen differentially expressed proteins in hPDMSCsLMP-1 were identified. They were potentially important participants in osteogenic differentiation, laid the foundation for further molecular mechanism elucidation of LMP-1 on hPDMSCs cell function and osteogenic differentiation.