口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (1): 1-4.DOI: 10.11839/kqyxzh.2016.01.001

• 基础研究论著 •    下一篇

MT01对Pg感染的成骨细胞MG63特异性成骨相关因子表达的影响

周岳,申玉芹,高涵,刘引,于海蛟,林崇韬*   

  1. 吉林大学口腔医院牙周病科 吉林 长春 130021
  • 收稿日期:2015-07-01 出版日期:2016-01-28 发布日期:2016-03-21
  • 通讯作者: 林崇韬,E-mail:linct@jl.u.edu.cn
  • 作者简介:周岳(1989~),女,吉林人,医师,硕士,主要从事牙周病学临床及基础的研究工作。
  • 基金资助:
    国家自然科学基金面上项目资助项目(编号:81371153)
    吉林省科技厅自然科学基金(编号:20150101173JC)

Effects of MT01 on the Expression of Specific Osteoblast-related Factors in MG63 Infected by Porphyromonas Gingivalis.

ZHOU Yue, SHEN Yu-qin, GAO Han, LIU Yin, YU Hai-jiao, LIN Chong-tao.   

  1. Department of Periodontology, Stomatological Hospital, Jilin University, Changchun 130021, China
  • Received:2015-07-01 Online:2016-01-28 Published:2016-03-21

PDF (PC)

175

摘要： 目的:通过检测MT01对牙周致病菌Pg感染的成骨细胞MG63细胞内特异性成骨相关因子基因表达水平,探讨MT01对感染状态下成骨细胞成骨分化作用的影响。方法:选取生长状态稳定的人成骨样细胞MG63接种于6孔板,分别加入质量浓度1 mg/L的特定序列寡核苷酸MT01和等体积PBS,共孵育3 h后,加入感染复数MOI=100∶1的Pg菌悬液(对照组加等体积PBS)。即实验分为:MT01+Pg、MT01、Pg和空白对照4组,Real-time PCR检测2、4、6、8、12、24 h特异性成骨相关因子Runx2,SP7 mRNA的表达。结果:在有无Pg感染的状态下,MT01均可上调成骨细胞MG63细胞内成骨相关因子Runx2,SP7 mRNA的表达,但在不同时间点,MT01调控Runx2,SP7 mRNA表达上调的能力存在差异。结论:MT01可以促进牙周致病菌感染下的成骨细胞的成骨向分化。

关键词: 特定序列寡核苷酸MT01, 牙龈卟啉单胞菌, 成骨相关因子, 人成骨样细胞MG63

Abstract: Objective: To explore the effects of MT01 on the differentiation of osteoblasts by determining the expression of specific osteoblast-related factors in MG63 infected by porphyromonas gingivalis. Methods: Human osteoblast-like cells MG63 were seeded into 6 well plates. Specific sequence oligodeoxynucleotide MT01 at a concentration of 1 μg·mL-1 and the same volume of PBS were added separately, and the cells were incubated for 3 hours. Then bacterium suspension of P.g at multiplicity of infection MOI=100∶1 was added. The same volume of PBS was added in the control group. Four groups were set, i.e. MT01+P.g, MT01, P.g, and blank control group. Real-time quantitative polymerase chain reaction was used to detect the mRNA expression of osteoblast-related factors Runx2, SP7 at 2, 4, 6, 8, 12, and 24 hours respectively. Results: The mRNA levels of osteoblast-related factors in MG63 were markedly upregulated after adding MT01 following infected by P.g or not. However, the ability of MT01 to upregulate the expression of Runx2 and SP7 at different time-points was different. Conclusion: MT01 could promote the differentiation of osteoblasts in infected status.

中图分类号: