整合素连接激酶和一氧化氮合酶在口腔鳞状细胞癌中的表达及意义

doi:10.13701/j.cnki.kqyxyj.2016.05.026

摘要/Abstract

摘要： 目的:探讨整合素连接激酶(ILK)和一氧化氮合酶(NOS)在口腔鳞状细胞癌(OSCC)中的表达及意义。方法:收集71例择期行手术切除治疗的OSCC患者肿瘤组织及癌旁组织,利用免疫组化SP法对ILK、eNOS和iNOS在肿瘤组织及癌旁组织中表达进行检测。结果:ILK、eNOS和iNOS在癌旁组织中阳性表达率分别为39.4%、45.1%和29.6%,且主要以弱阳性为主,而在OSCC组织中阳性表达率分别为98.6%、93.0%和95.8%,且以强阳性表达为主,ILK、eNOS和iNOS在OSCC组织中阳性表达显著高于癌旁组织,差异均有统计学意义(P<0.05);ILK和iNOS在OSCC组织中表达均与分化程度和淋巴结转移有关(P<0.05);Spearman相关分析显示,ILK在OSCC组织中强表达与iNOS强表达呈正相关(r=0.529,P=0.000)。结论:ILK、eNOS和iNOS在OSCC组织中出现高表达,可能通过促进肿瘤中血管生成而参与OSCC细胞浸润和转移过程。

关键词: 口腔鳞状细胞癌, 整合素连接激酶, 一氧化氮合酶, 表达

Abstract: Objective: To investigate the expression and significance of integrin-linked kinase (ILK) and nitric oxide synthase (NOS) in oral squamous cell carcinoma (OSCC). Methods: 71 cases of tumor and adjacent tissues of patients with OSCC undergoing surgical resection were collected. The expression of ILK, eNOS and iNOS in tumor and adjacent tissues were detected by immunohistochemistry. Results: The positive expression rates of ILK, eNOS and iNOS in adjacent tissues were 39.4%, 45.1% and 29.6%, and most of them were weakly positive, while in OSCC tissues were 98.6%, 93.0% and 95.8%, and most of them were strongly positive. The expression levels of ILK, eNOS and iNOS in OSCC tissues were significantly higher than the adjacent tissues (P<0.05). The expression levels of ILK and iNOS in OSCC tissues were correlated with the degree of cell differentiation and lymph node metastasis (P<0.05). Spearman correlation analysis showed that the expression of ILK in OSCC tissues was positively correlated with the expression of iNOS (r=0.529, P=0.000). Conclusion: Up-regulated expression of ILK, eNOS and iNOS in OSCC might be involved in the cell invasion and metastasis through promoting tumor angiogenesis.

中图分类号:

R739.8

管永昱. 整合素连接激酶和一氧化氮合酶在口腔鳞状细胞癌中的表达及意义[J]. 口腔医学研究, 2016, 32(5): 527-530.

GUAN Yong-yu. Expression and Significances of Integrin-linked Kinase and Nitric Oxide Synthase in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2016, 32(5): 527-530.

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