口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (6): 580-583.DOI: 10.13701/j.cnki.kqyxyj.2016.06.007

• 临床研究论著 • 上一篇    下一篇

MT01对感染状态人成骨样细胞内ALP活性及mRNA表达的影响

高涵1,2 ,申玉芹1 ,刘引1,2 ,胡天琦1,2 ,费鸿博1,2 ,顾中一1,2 ,李洋洋1,2 ,林崇韬1*   

  1. 1. 吉林大学口腔医学院牙周病科 吉林 长春 130021;
    2. 吉林省牙发育及颌骨重塑与再生重点实验室 吉林 长春 130021
  • 收稿日期:2015-12-28 出版日期:2016-06-26 发布日期:2016-06-22
  • 通讯作者: 林崇韬,E-mail:linct@jl.u.edu.cn
  • 作者简介:高涵(1991~ ),女,吉林省榆树市人,硕士,主要从事牙周病学的研究工作。
  • 基金资助:
    国家自然科学基金面上项目资助项目(编号:81371153)
    吉林省科技厅自然科学基金(20150101173JC)

Effects of MT01 on the ALP Activity and mRNA Expression of MG63 Infected by Porphyromonas Gingivalis.

GAO Han1,2, SHEN Yu-qin1, LIU Yin1,2, HU Tian-qi1,2, FEI Hong-bo1,2, GU Zhong-yi1,2, LI Yang-yang1,2, LIN Chong-tao1*.   

  1. 1. Dept. of Periodontology, School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
    2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China
  • Received:2015-12-28 Online:2016-06-26 Published:2016-06-22

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摘要： 目的:通过检测MT01对牙龈卟啉单胞菌感染的人成骨样细胞内特异性成骨相关因子ALP活性及mRNA表达水平的改变,探讨MT01对感染状态下人成骨样细胞成骨向分化的影响。方法:选取状态良好的MG63细胞接种于6孔板内,2个MT01组加入质量浓度为1mg/L的MT01,共孵育3h后,相应组加入感染复数为100∶1的Pg菌悬液。实验分为:空白对照、MT01、Pg和MT01+Pg4组,碱性磷酸酶测试盒测定24h后上清液及细胞内ALP活性。Real-timePCR检测2、4、6、8、12、24h特异性成骨相关因子ALPmRNA的表达。结果:在感染与非感染情况下,MT01均可促进ALP活性,且上调MG63细胞内成骨相关因子ALPmRNA表达,其表达上调呈时间依赖性。结论:MT01可促进感染及非感染状态下MG63内ALP基因表达水平,提高ALP活力。

关键词: 人成骨样细胞MG63, 牙龈卟啉单胞菌, 特定序列寡核苷酸MT01, 碱性磷酸酶

Abstract: Objective: To study the effect of MT01 on the differentiation of osteoblasts infected by porphyromonas gingivalis (Pg). Methods: The MG63 cells were seeded into 6- well cell culture plates. MT01 at a final concentration of 1 μg·mL-1 was added and incubated for 3 hours. Then the cells were challenged by Pg (MOI=100∶1). There were four experimental groups: blank group, MT01, Pg and MT01+Pg. ALP activities in the supernatant fluid and the cell lysate after 24 hours were detected by ALP kit. Real-time PCR was used to detect the mRNA expression of ALP after 2, 4, 6, 8, 12, and 24 hours. Results: MT01 enhanced the ALP activity in infected or uninfected conditions. The mRNA expression of ALP in MG63 was upregulated by MT01 post-infected by Pg or not in a time-dependent manner. Conclusion: MT01 can upregulate the mRNA expression of ALP in Pg infected or uninfected MG63 and improve the ALP activity.

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