口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (5): 486-490.DOI: 10.13701/j.cnki.kqyxyj.2017.05.006

• 基础研究论著 • 上一篇    下一篇

SMAS修饰的PEGDA载电凝胶对MC3T3-E1细胞粘附和增殖的影响

曲福祯1,2,谈飞1,刘杰1,2*   

  1. (1. 青岛大学附属医院口腔修复科 山东 青岛 266003;2. 青岛大学口腔医学院 山东 青岛 266003)
  • 收稿日期:2016-10-28 出版日期:2017-05-20 发布日期:2017-05-26
  • 通讯作者: 刘杰,E-mail:18661801995@163.com
  • 作者简介:曲福祯(1991~ ),女,山东人,硕士在读,主要从事口腔修复的临床及科研工作。
  • 基金资助:
    山东省2014年科学技术发展计划医药卫生项目(编号:2014GSF121011)

Effect of SMAS Modified PEGDA Hydrogel on Attachment and Proliferation of MC3T3-E1 Cells.

QU Fu-zhen1,2, TAN Fei1, LIU Jie1,2*.   

  1. 1. Department of Prosthodontics, The Affiliated Hospital of Qingdao University, Qingdao 266003, China; 2. School of Stomatology, Qingdao University, Qingdao 266003, China.
  • Received:2016-10-28 Online:2017-05-20 Published:2017-05-26

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摘要： 目的:观察甲基丙烯磺酸钠(SMAS)修饰的聚乙二醇双丙烯酸酯(PEGDA)水凝胶对小鼠前成骨细胞(MC3T3-E1细胞)粘附及增殖的影响。方法:通过在PEGDA凝胶前体溶液中加入不同浓度的SMAS小分子,合成出携带不同电量负电荷的新型载电凝胶。扫描电镜观察各组水凝胶的表面结构。检测各组水凝胶的蛋白吸附量。观察种子细胞在各组水凝胶表面的早期粘附,并检测不同时间点下凝胶表面细胞粘附量。用cck8法检测细胞在凝胶表面的增殖情况。结果:随着SMAS浓度的提高,凝胶的蛋白吸附量不断提高,同时随着SMAS修饰量的提高,细胞在凝胶表面得到更好的伸展,同时凝胶表面的细胞粘附数明显提高(P<0.05)。而在细胞增殖方面,cck8结果表明随着SMAS修饰量的提高,凝胶表面的细胞增殖也得到明显改善。结论: SMAS小分子修饰的PEGDA凝胶能够有效地促进MC3T3-E1细胞在其表面的粘附和增殖。

关键词: 骨组织工程, 载电凝胶, 负电荷

Abstract: Objective: To investigate the effect of sodium methylallyl sulfonate (SMAS) modified poly(ethylene glycol) diacrylate (PEGDA) hydrogel on the attachment and proliferation of osteoblast-like cells (MC3T3-E1). Methods: A series of negative charged hydrogels were fabricated by incorporating charged monomer (SMAS) into PEGDA hydrogel at different concentrations. Scanning electron microscopy (SEM) was used to observe the surface morphology of the hydrogels. The protein adsorption of the different hydrogels was evaluated by BCA. To evaluate the cell attachment and proliferation on the hydrogel, the MC3T3-E1 cells were seeded onto the surface of hydrogels. Then, the cell morphology was observed at different time points. Furthermore, the cell proliferation was investigated by cell counting kit (CCK-8). Results: With increasing concentration of SMAS, the protein adsorption of the hydrogel was significantly enhanced (P<0.05). Meanwhile, compared to control group, the cell attachment of HG100 group and HG200 group were improved. The results of CCK8 assay showed that the cell proliferation was promoted on the modified hydrogels. Conclusion: The SMAS modified hydrogel can effectively promote the cell attachment and proliferation of MC3T3-E1 cells.

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