慢性睡眠剥夺对大鼠髁突MMP-2和MMP-9表达的影响

doi:10.13701/j.cnki.kqyxyj.2021.11.011

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (11): 1009-1016.DOI: 10.13701/j.cnki.kqyxyj.2021.11.011

慢性睡眠剥夺对大鼠髁突MMP-2和MMP-9表达的影响

刘阳¹, 白月辉¹, 崔玉兰^1,2,3, 赵琛^1,2,3*

1.河北医科大学口腔医学院·口腔医院修复科河北石家庄 050017;
2.河北省口腔医学重点实验室河北石家庄 050017;
3.河北省口腔疾病临床医学研究中心河北石家庄 050017

收稿日期:2021-05-06 出版日期:2021-11-28 发布日期:2021-11-22
通讯作者: *赵琛,E-mail:zhaochen36@163.com
作者简介:刘阳(1992~ ),男,河北承德人,医师,硕士在读,研究方向:颞下颌关节紊乱病。

Effects of Chronic Sleep Deprivation on Expression of MMP-2 and MMP-9 in Condyle of Rats

LIU Yang¹, BAI Yuehui¹, CUI Yulan^1,2,3, ZHAO Chen^1,2,3*

1. Department of Prosthodontics, School and Hospital of Stomatology, Hebei Medical University, Shijiazhuang 050017, China;
2. Hebei Key Laboratory of Stomatology, Shijiazhuang 050017, China;
3. Hebei Clinical Research Center For Oral Disease, Shijiazhuang 050017, China

Received:2021-05-06 Online:2021-11-28 Published:2021-11-22

摘要/Abstract

摘要： 目的: 探讨慢性睡眠剥夺及睡眠恢复对大鼠颞下颌关节髁突基质金属蛋白酶(MMP)-2、MMP-9表达的影响。方法: 利用改良多平台法(MMPM)法对大鼠施加为期1、2、3、4周时间的慢性睡眠剥夺,并利用旷场实验进行检验,通过HE染色观察实验组、对照组和恢复组大鼠髁突软骨组织的结构变化,通过免疫组织化学方法检测各组大鼠髁突MMP-2、MMP-9的表达变化。结果: 旷场实验中各实验组及恢复组在结束慢性睡眠剥夺时水平得分与垂直得分均高于对照组大鼠,差异具有统计学意义(P<0.05)。恢复组经过1周时间睡眠恢复后水平得分与垂直得分与对照组无明显差异(P>0.05)。HE染色观察发现,慢性睡眠剥夺后实验组大鼠髁突软骨出现了退行性表现。恢复组与实验组相比,软骨组织出现修复性增生改变。免疫组织化学结果显示:MMP-2、MMP-9在各时间点实验组中的阳性表达强度高于对照组(P<0.05),各时间点恢复组阳性表达强度低于实验组(P<0.05)。结论: 慢性睡眠剥夺可引起大鼠髁突软骨退行性改变,软骨细胞分泌的MMP-2、MMP-9参与了其病理改变的进程。

关键词: 慢性睡眠剥夺, 颞下颌关节, 髁突软骨, 基质金属蛋白酶-2, 基质金属蛋白酶-9

Abstract: Objective: To investigate the effects of chronic sleep deprivation and sleep recovery on the development of temporomandibular joint degeneration in rats and the changes in the expression of MMP-2 and MMP-9. Methods: MMPM method was used to establish the rat model of chronic sleep deprivation for 1, 2, 3, and 4 weeks and the open field test was used to verify. The HE staining was used to observe the changes of rat’s condylar cartilage tissue and immunohistochemical method was used to detect the expression of MMP-2 and MMP-9 in condylar cartilage. Results: MMPM could successfully induce the rats into the chronic sleep deprivation state. HE staining showed that the experimental group’s condyle cartilage had degenerative changes. Immunohistochemical results showed that the positive expression intensity of MMP-2 and MMP-9 in the experimental group at each time point was higher than that in the control group (P<0.05), and the positive expression intensity in the recovery group at each time point was lower than that in the experimental group (P<0.05). Conclusion: Chronic sleep deprivation can cause degeneration changes of condyle cartilage in rats, and the secretion of MMP-2 and MMP-9 by chondrocytes is involved in the degeneration process.

Key words: chronic sleep deprivation, temporomandibular joint, condylar cartilage, matrix metalloproteinase-2, matrix metalloproteinase-9

刘阳, 白月辉, 崔玉兰, 赵琛. 慢性睡眠剥夺对大鼠髁突MMP-2和MMP-9表达的影响[J]. 口腔医学研究, 2021, 37(11): 1009-1016.

LIU Yang, BAI Yuehui, CUI Yulan, ZHAO Chen. Effects of Chronic Sleep Deprivation on Expression of MMP-2 and MMP-9 in Condyle of Rats[J]. Journal of Oral Science Research, 2021, 37(11): 1009-1016.

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慢性睡眠剥夺对大鼠髁突MMP-2和MMP-9表达的影响

Effects of Chronic Sleep Deprivation on Expression of MMP-2 and MMP-9 in Condyle of Rats

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