口腔医学研究

口腔医学研究 ›› 2025, Vol. 41 ›› Issue (12): 1050-1054.DOI: 10.13701/j.cnki.kqyxyj.2025.12.006

• 牙周病学研究 • 上一篇    下一篇

淫羊藿苷调节NOX2/ROS/NF-κB通路对牙周炎大鼠牙周组织炎性损伤的影响

肖遵胜1*, 丁文萃2, 刘璐1, 温超3   

  1. 1.沧州市中心医院口腔门诊 河北 沧州 061001;
    2.沧州市中心医院内分泌糖尿病一科 河北 沧州 061001;
    3.沧州市中心医院输血科 河北 沧州 061001
  • 收稿日期:2025-04-01 出版日期:2025-12-28 发布日期:2025-12-23
  • 通讯作者: *肖遵胜,E-mail:s19hdw@163.com
  • 作者简介:肖遵胜(1983~ ),男,河北沧州人,硕士,副主任医师,研究方向:口腔医学。
  • 基金资助:
    河北省2025年度医学科学研究课题计划项目(编号:20251518)

Impact of Icariin on Periodontal Tissue Inflammatory Damage in Periodontitis Rats by Regulating NOX2/ROS/NF-κB Pathway

XIAO Zunsheng1*, DING Wencui2, LIU Lu1, WEN Chao3   

  1. 1. Oral Clinic of Cangzhou Central Hospital, Cangzhou 061001, China;
    2. Department of Secretory Diabetes, Cangzhou Central Hospital, Cangzhou 061001, China;
    3. Department of Blood Transfusion, Cangzhou Central Hospital, Cangzhou 061001, China
  • Received:2025-04-01 Online:2025-12-28 Published:2025-12-23

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摘要： 目的: 探讨淫羊藿苷(icariin,ICA)调节NADPH氧化酶2(reduced nicotinamide adenine dinucleotide phosphate oxidase 2,NOX2)/活性氧(reactive oxygen species,ROS)/核因子-κB(nuclear factor-κB,NF-κB)通路对牙周炎大鼠牙周组织炎性损伤的影响。方法: 将大鼠分为对照组、模型组、ICA低剂量组(ICA-L,20 mg/kg)、ICA高剂量组(ICA-H,40 mg/kg)和NOX2抑制剂组(GSK2795039,2.5 mg/kg),每组12只。利用牙龈出血指数(bleeding index,BI)评估牙周组织炎症情况;Micro-CT检测牙槽骨吸收;苏木精-伊红染色 (hematoxylin-eosin staining, HE)检测牙周组织病理形态;抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphase,TRAP)染色检测破骨细胞数;ELISA法检测白细胞介素-1β(interleukin-1β,IL-1β)、干扰素-γ(interferon-γ,IFN-γ)、超氧化物歧化酶(superoxide dismutase,SOD)、ROS、过氧化氢酶(catalase,CAT)水平;Western blot检测NOX2和NF-κB蛋白表达。结果: 与对照组比较,模型组大鼠BI、釉牙骨质界至牙槽嵴顶(cementoenamel junction and alveolar bone crest,CEJ-ABC)距离、IL-1β、IFN-γ、ROS水平、NOX2和NF-κB表达升高(P＜0.05),SOD和CAT水平显著下降(P＜0.05),牙龈上皮糜烂,牙龈固有层炎症细胞浸润,牙槽嵴顶下移,有大量活跃的破骨细胞;与模型组比较,ICA-L、ICA-H组大鼠BI、CEJ-ABC距离、IL-1β、IFN-γ、ROS水平、NOX2和NF-κB蛋白表达显著升高(P＜0.05),SOD和CAT表达水平显著下降(P＜0.05),牙龈上皮遭到破坏,炎性细胞浸润显著减少,牙槽嵴顶明显上移,破骨细胞减少。GSK2795039处理可逆转ICA对上述指标的影响。结论: ICA可能通过抑制NOX2/ROS/NF-κB通路改善牙周炎大鼠牙周组织炎性损伤。

关键词: 淫羊藿苷, NADPH氧化酶2/活性氧/核因子-κB通路, 牙周炎, 牙周组织, 炎性损伤

Abstract: Objective: To discuss the impact of icariin (ICA) on periodontal tissue inflammatory damage in periodontitis rats by regulating NADPH oxidase 2 (NOX2)/reactive oxygen species (ROS)/nuclear factor kappa B (NF-κB) pathway. Methods: Rats were grouped into control group, model group, ICA low-dose group (ICA-L, 20 mg/kg), ICA high-dose group (ICA-H, 40 mg/kg), and NOX2 inhibitor group (2.5 mg/kg), each with 12 rats. Gingival bleeding index (BI) was used to assess periodontal tissue inflammation. Micro CT was used to measure alveolar bone resorption. HE staining was used to measure the pathological morphology of periodontal tissue. Tartrate resistant acid phosphatase (TRAP) staining was used to detect osteoclast count. ELISA method was used to test interleukin-1β (IL-1β), interferon-γ (IFN-γ), superoxide dismutase (SOD), ROS, and catalase (CAT). Western blot was used to detect the NOX2 and NF-κB proteins. Results: Compared with the control group, the model group showed prominent increases in BI, CEJ-ABC distance, IL-1β, IFN-γ, ROS levels, NOX2, and NF-κB proteins (P<0.05), significant decreases in SOD and CAT (P<0.05), ulceration and erosion of gingival epithelium, extensive infiltration of inflammatory cells in the gingival solid layer, a prominent downward movement of the alveolar ridge, and a large number of active osteoclasts. Compared with the model group, the ICA-L group, ICA-H group, and NOX2 inhibitor group showed prominent increases in GI, CEJ-ABC distance, IL-1β, IFN-γ, ROS levels, NOX2, and NF-κB proteins (P<0.05), significant decreases in SOD and CAT (P<0.05), obvious reduction in gingival epithelium damage and inflammatory cell infiltration, upward movement of alveolar ridge crest, and decrease in osteoclasts. GSK2795039 treatment could reverse the effects of ICA on the above indicators. Conclusion: ICA may improve the periodontal tissue inflammatory damage in periodontitis rats by inhibiting NOX2/ROS/NF-κB pathway.

Key words: icariin, NOX2/ROS/NF-κB pathway, periodontitis, periodontal tissue, inflammatory injury