Amelotin基因对结合上皮屏障功能影响的研究

doi:10.13701/j.cnki.kqyxyj.2026.07.004

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (7): 569-574.DOI: 10.13701/j.cnki.kqyxyj.2026.07.004

Amelotin基因对结合上皮屏障功能影响的研究

褚青¹, 牟海瑜², 许针针¹, 孙轶群¹, 高艳¹, 高玉光¹, 相黎黎¹, 董志恒^1*

1.山东医药大学附属医院儿童口腔科山东滨州 256600;
2.青岛大学附属烟台毓璜顶医院口腔科山东烟台 264000

收稿日期:2025-10-15 发布日期:2026-07-22
通讯作者: *董志恒,E-mail:dzh1987.good@163.com
作者简介:褚青(1989~ ),女,山东枣庄人,硕士,研究方向:牙齿发育的分子机制研究。
基金资助:
国家自然科学基金(编号:82100999) 滨州医学院“临床+X”科技创新项目(编号:BY2021LCX19) 滨州医学院大创项目(编号:X2025104400979)

Impact of Amelotin Gene on Barrier Function of Junctional Epithelium

CHU Qing¹, MU Haiyu², XU Zhenzhen¹, SUN Yiqun¹, GAO Yan¹, GAO Yuguang¹, XIANG Lili¹, DONG Zhiheng^1*

1. Department of Pediatric Dentistry, Shandong Medical and Pharmaceutical University Hospital, Binzhou 256600, China;
2. Department of Stomatology, Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China

Received:2025-10-15 Published:2026-07-22

摘要/Abstract

摘要： 目的: 探讨amelotin敲除(Amtn^-/-)对结合上皮屏障(junctional epithelium barrier, JEB)功能的影响。方法: 对比同窝Amtn^-/-小鼠与野生型(wild type, WT)小鼠颌骨标本,通过扫描电镜(scanning electron microscope, SEM)、苏木精-伊红(hematoxylin-eosin,HE)染色、免疫组织化学检测(immunohistochemistry, IHC)及微计算机断层扫描(micro-computed tomography, micro-CT)分析牙周组织及釉质矿化情况。结果: 3月龄Amtn^-/-小鼠出现牙槽骨吸收、根分叉暴露,牙周纤维紊乱、结合上皮(junctional epithelium, JE)断裂并向根方迁移,且随年龄增长而加剧。1月龄Amtn^-/-小鼠磨牙釉质磨损严重,成釉细胞基质蛋白残留,未萌出磨牙Amtn^-/-鼠缺乏上皮覆盖。对于Amtn^-/-鼠,IHC显示层粘连蛋白亚基γ2(laminin gamma-2,LAMC2)在JE基板表达下调、JE内部上调;基质金属蛋白酶-9(matrix metalloproteinase-9, MMP-9)在JE基板和内部均增强。结论: Amtn基因缺失可引发釉质矿化不良、JE粘附破坏,可能与LAMC2和MMP-9异常表达有关。

关键词: Amelotin, 釉质发育不良, 结合上皮屏障, 上皮附着, 牙周病

Abstract: Objective: To investigate the impact of amelotin knockout (Amtn^-/-) on the function of the junctional epithelium barrier (JEB). Methods: Mandibular specimens from littermate Amtn^-/- and wild-type (WT) mice were compared. Periodontal tissue morphology and enamel mineralization were analyzed using scanning electron microscopy (SEM), hematoxylin-eosin (HE) staining, immunohistochemistry (IHC), and micro-computed tomography (micro-CT). Results: Three-month-old Amtn^-/- mice exhibited alveolar bone resorption, furcation exposure, disorganized periodontal fibers, junctional epithelium (JE) disintegration with apical migration, and age-dependent exacerbation. One-month-old Amtn^-/- mice showed severe molar enamel wear, residual matrix proteins in ameloblasts, and lack of epithelial coverage in unerupted molars (Amtn^-/- mice). For Amtn^-/- mice, IHC revealed downregulated laminin gamma-2 (LAMC2) expression in the JE basement membrane but upregulated within JE; matrix metalloproteinase-9 (MMP-9) expression was enhanced in both basement membrane and JE interior. Conclusion: Deletion of the Amtn gene can lead to poor enamel mineralization and destruction of JE adhesion, which may be related to the abnormal expression of LAMC2 and MMP-9.

Key words: Amelotin, amelogenesis imperfecta, junctional epithelial barrier, epithelial attachment, periodontal disease

褚青, 牟海瑜, 许针针, 孙轶群, 高艳, 高玉光, 相黎黎, 董志恒. Amelotin基因对结合上皮屏障功能影响的研究[J]. 口腔医学研究, 2026, 42(7): 569-574.

CHU Qing, MU Haiyu, XU Zhenzhen, SUN Yiqun, GAO Yan, GAO Yuguang, XIANG Lili, DONG Zhiheng. Impact of Amelotin Gene on Barrier Function of Junctional Epithelium[J]. Journal of Oral Science Research, 2026, 42(7): 569-574.

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Amelotin基因对结合上皮屏障功能影响的研究

Impact of Amelotin Gene on Barrier Function of Junctional Epithelium

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