口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (2): 133-136.

• 临床研究论著 • 上一篇    下一篇

慢性牙周炎唾液牙龈卟啉单胞菌的定量PCR检测

袁杰1,王少果1,周建业2,吾斯曼江艾尔肯1,何祥一1,李志强3*   

  1. 1. 兰州大学口腔医学院牙周科 甘肃 兰州 730000;
    2. 兰州大学土木工程与力学学院;
    3. 西北民族大学 口腔医学院
  • 收稿日期:2014-06-20 出版日期:2015-02-28 发布日期:2016-04-29
  • 通讯作者: 李志强,E-mail: lizhiqiang6767@163.com
  • 作者简介:袁杰(1986~ ),女,山东聊城人,硕士,主要从事口腔微生物学方向的研究。
  • 基金资助:
    国家自然科学基金项目(31160124/C0309、31360124/ C0309) 甘肃省自然科学基金(1308RJZA248, 1204FKCA168)资助

Quantitative PCR Detection of Porphyromonas Gingivalis of Chronic Periodontitis in Saliva.

YUAN Jie, WANG Shao-guo, ZHOU Jian-ye, et al   

  1. School of Stomatology, Lanzhou University, Lanzhou 730000
  • Received:2014-06-20 Online:2015-02-28 Published:2016-04-29

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摘要： 目的:定量检测慢性牙周炎患者、牙周健康人群唾液中牙龈卟啉单胞菌的含量,比较其在各组人群分布的差异。方法:应用SYBR Green模式的实时荧光定量PCR技术,针对Pg特异基因Arg-gingipain设计引物,检测20例慢性牙周炎和20例牙周健康者唾液内Pg的含量,t检验分析比较在各组人群中Pg定植的差异。结果:慢性牙周炎患者唾液中,Pg的检出数目为(1.78×103~1.99×105),检出率为85%;牙周健康者唾液中,Pg的检出数目为(2.19×103~2.30×103),检出率为10%。Pg在慢性牙周炎和牙周健康者唾液中检出数目和检出率的差异具有统计学意义(P<0.05)。结论:慢性牙周炎唾液中,牙龈卟啉单胞菌较正常人群明显升高,在今后的研究及防治中,不仅要观察龈沟液中的牙龈卟啉单胞菌,也应注意其在唾液中含量的变化。

关键词: 实时荧光定量PCR, 慢性牙周炎, 牙龈卟啉单胞菌, 唾液

Abstract: Objective: To detect the amount of Pg in saliva of periodontitis patients and periodontal healthy subjects, then assess differences in the Pg number between the groups. Methods: We examined quantity of Pg in saliva of 20 chronic periodontitis and 20 periodontal health by Pg specific genes Arg-gingipain adopting SYBR Green real-time fluorescent quantitative PCR technology. T test was used to analyze the differences of Pg engraftment among all groups. Results: In samples of chronic periodontitis, the number of Pg was 1.78×103-1.99×105 and positive rates was 85%; While in samplels with periodontal healthy subjects, the number of Pg was 2.19×103-2.30×103,and positive rates was 10%. The difference of saliva Pg number between chronic periodontitis and periodontal healthy subjects was significant in statisties (P＜0.05). Conclusion: The number of Pg in chronic periodontitis saliva increased highly than in periodontal healthy subjects. In the future research and prevention, not only Pg in the gingival crevicular fluid should be detected, but also we should pay attention to it in the saliva.

Key words: Real-time fluorescent quantitative , PCR , Chronic periodontitis , Porphyromonas gingivalis, Saliva

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