种植体周围黏膜炎龈下微生物群落多样性研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 719-723.

种植体周围黏膜炎龈下微生物群落多样性研究

陈新¹,熊萍²,钟科^3*,孙勇^3*,陈一⁴

1. 重庆医科大学附属永川医院口腔科重庆 610011;
2. 德阳市第二人民医院口腔科;
3. 成都军区机关医院口腔科;
4. 南充市中心医院口腔科

收稿日期:2014-04-22 出版日期:2015-07-28 发布日期:2015-07-28
通讯作者: 钟科,电话:13808008012;孙勇,电话:13808008089
作者简介:陈新(1987~ ),女,重庆人,硕士在读,医师,研究方向是种植修复。

Study on the Diversity of Subgingival Microflora in Implant Mucositis

CHEN Xin, XIONG Ping, ZHONG Ke, et al

Department of Stomatology, Affiliated Yongchuang Hospital, Chongqing Medical University. Chongqing 610011

Received:2014-04-22 Online:2015-07-28 Published:2015-07-28

摘要/Abstract

摘要： 目的:检测种植体周围黏膜炎龈下微生物群落的多样性,研究种植体周围黏膜炎龈下微生物种类、数量以及群落分布,找出特异性微生物,为临床上选择适当的维护与干预方法提供依据。方法:采集10例患黏膜炎的种植体龈沟液样本(A组)及14例健康种植体的龈沟液样本(B组),提取样本龈沟液中的微生物总DNA作为模板,采用细菌的16S rDNA V3区通用引物进行扩增,扩增产物进行DGGE电泳分析及克隆测序分析。结果:A组较B组龈沟液量多,差异有显著统计学意义(P﹤0.01)。AB两组样本整体相似性为18%,A组各样本群落相似性为29.9%~60.2%,B组各样本相似性30.8%~74%。A组测得5种菌门,包括15种菌属,B组测得4种菌门,12种菌属。A组G^-厌氧杆菌比例为69.65%,B组为64.51%。结论:发生种植体周围黏膜炎时龈沟液量较健康种植体增加,龈沟液量可一定程度上反映种植体周围软组织的健康状况。种植体周围组织在健康状态和发生黏膜炎时的龈沟液中微生物群落均具有高度多样性,但菌群结构差异较大。健康种植体及种植体周围黏膜炎龈下微生物群落均以G^-厌氧杆菌为主,后者G^-厌氧杆菌比例更高。

关键词: 种植体, 聚合酶链反应-变性梯度凝胶电泳, 16S, rDNA, 微生物多样性

Abstract: Objective: To explore the diversity of subgingival microflora in implant mucositis and help to choose proper maintenance and intervention method. Methods: Microbial total DNA of gingival crevicular fluid samples from 10 cases which had been diagnosed as implant mucositis and 14 healthy implants. PCR (polymerase chain reaction) was conducted with bacteria 16S rDNA-V3 region as primer. The amplification products were analyzed using DGGE (denaturing gradient gel electrophoresis) and the main DNA bands of DGGE were sliced and used for sequence analysis. Results: Significant statistical differences were observed between group A and group B on PICF index (P<0.01). UPGMA cluster analysis of two groups showed 18% similarity overall. Group A:The similarity ranged from 29.9% to 60.2%; group B: the similarity ranged from 30.8% to 74%. Five phylums were detected in group A, including fifteen genus, which contained an uncultured bacterium, and Candidate division TM7 phylum was found only in this group; Four phylums were detected in group B, including twelve genus. Conclusion: Gingival crevicular fluid volume increased in peri-implant mucositis when compared with healthy implants. Microbial communities of samples in both healthy implants and implants with mucositis showed high diversities, while showed big difference in microbial community structures. The proportion of gram negative anaerobic bacillus increased as peri-implant mucositis occurred.

Key words: Implant, PCR-DGGE, 16S rDNA, Microbial diversity

中图分类号:

R781.4

陈新,熊萍,钟科,孙勇,陈一. 种植体周围黏膜炎龈下微生物群落多样性研究[J]. 口腔医学研究, 2015, 31(7): 719-723.

CHEN Xin, XIONG Ping, ZHONG Ke, et al. Study on the Diversity of Subgingival Microflora in Implant Mucositis[J]. Journal of Oral Science Research, 2015, 31(7): 719-723.

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