口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (8): 779-.

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白细胞介素-24通过Jak-Stat通路对破骨细胞影响的实验研究

孟庆阳1,孙宏晨2,史册2,郑嵘1,李超1,孟艾奇1,张布1,吴立鹏1*   

  1. 1. 佳木斯大学口腔医学院正畸科 黑龙江 佳木斯 154000;
    2. 吉林大学口腔医学院
  • 收稿日期:2015-03-09 出版日期:2015-08-28 发布日期:2016-05-04
  • 通讯作者: 吴立鹏,Email:WLPKQ@sina.com
  • 作者简介:孟庆阳(1987~ ),男,黑龙江人,硕士,医师,主要从事口腔正畸的临床治疗工作。
  • 基金资助:
    佳木斯大学研究生创新科研项目(编号:LZZ2014-025)

Effect of IL-24 on Osteoclasts through Jak-Stat Signaling Pathway

MENG Qing-yang, SUN Hong-chen, SHI Ce, et al   

  1. School of Stomatology, Jiamusi University, Jiamusi 154000
  • Received:2015-03-09 Online:2015-08-28 Published:2016-05-04

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摘要： 目的:研究白细胞介素-24(interleukin-24,IL-24)在体外通过Jak-Stat信号通路对单核巨噬细胞向破骨细胞分化的影响。方法:使用增强型绿色荧光蛋白(EGFP)确定最佳感染复数(MOI);使用ELISA检测培养基中IL-24的含量;采用Real-time PCR方法检测RAW264.7细胞中Jak-Stat信号通路相关基因Jak1、Jak2、Jak3、Stat1、Stat2、Stat3及其向破骨细胞分化过程中相关基因NFATc1、CTSK、MMP9 mRNA的表达。结果:MOI=600为最佳转染效率;转染组IL-24含量显著高于未转染组(P＜0.01);在RANKL诱导条件下转染IL-24的RAW264.7细胞与未转染组相比Jak2、Stat3 mRNA的表达增加(P＜0.05),在RANKL诱导条件下转染IL-24的RAW264.7细胞加入信号通路抑制剂后与未加入抑制剂组相比,向破骨细胞分化过程中相关基因NFATc1、CTSK、MMP9 mRNA的表达降低(P＜0.05)。结论:IL-24通过Jak-Stat信号通路调控破骨细胞的分化及功能。

关键词: 破骨细胞, RAW264.7, 骨吸收, 白细胞介素24, 信号传导

Abstract: Objective: To study the effect of IL-24 on the differentiation and function of osteoclasts through Jak-Stat signaling pathway. Methods: The enhanced green fluorescent protein (EGFP) carried by adenovirus was used to determine the optimal multiplicity of infection (MOI). ELISA was used to detect the content of IL-24 in the medium. Real-time PCR was used to detect the related genes of Jak-Stat signaling pathway such as Jak1, Jak2, Jak3, Stat1, Stat2 and Stat3 and the expression of osteoclastic factor in RANKL-induced RAW264.7 such as NFATc1, CTSK and MMP9. Results: MOI=600 was the best condition. Content of IL-24 in the medium of transfected group was higher than that of vector group. Under the induction of RANKL, compared with vector group, the transfected group expressed higher level of Jak2 and Stat3 (P＜0.05). The addition of Stattic decreased the expression of NFATc1, CTSK and MMP9 for RAW264.7 cells transfected with IL-24 gene (P＜0.05). Conclusion: IL-24 could promote the differentiation and function of osteoclasts through Jak-Stat signaling pathway.

Key words: Osteoclast, RAW264.7, Bone resorption, Interleukin-24, Signaling transduction

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